Thiazolyl Blue (solution)  (Synonyms: MTT (solution); Thiazolyl Blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-tetrazolium bromide (solution))

Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis^[1].
Solvent and concentration: PBS: 5 mg/mL

Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
1. Cell Proliferation Assay (96-Well Plate)
1.1 Cell seeding: Prepare a single-cell suspension in culture medium containing 10% FBS and seed into a 96-well plate at 1000-10000 cells per well in a final volume of 100 μL per well.
1.2 Cell culture: Incubate cells at 37°C with 5% CO₂ for 24-72 h.
1.3 Add 10 μL MTT to each well and incubate for 4 h. Discard the supernatant; for suspension cells, centrifuge before removing the supernatant.
1.4 Add 100 μL DMSO and shake for 10 minutes to completely dissolve the crystals.
1.5 Measure the increase in absorbance at OD=562 nm using a microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

414.32

C₁₈H₁₆BrN₅S

298-93-1

CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.  [Content Brief]

  [2]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.  [Content Brief]