CircAP2A2 acts as a ceRNA to participate in infantile hemangiomas progression by sponging miR-382-5p via regulating the expression of VEGFA

  • J Clin Lab Anal. 2020 Jul;34(7):e23258. doi: 10.1002/jcla.23258.
Xiaoqi Yuan  1 ,  Yanan Xu  1 ,  Zhiqiang Wei  1 ,  Qi Ding  2
Affiliations
  • 1. The Ningbo Women and Children's Hospital, Ningbo, China.
  • 2. Department of Diagnosis, Ningbo Diagnostic Pathology Center, Ningbo, China.
PMID: 32091151 DOI: 10.1002/jcla.23258
Abstract

Background: Increasing evidences reveal that circular RNAs (circRNAs) play crucial functions in Cancer development. However, the expression pattern and roles of circRNAs in infantile hemangiomas (IH) remain unclear.

Methods: In this study, qRT-PCR was performed to determine the expression of circAP2A2, miR-382-5p, and VEGFA in IH tissues and cell lines. Moreover, MTT assay, colony formation, transwell assay, and Western blot analysis were conducted to assess the function of circAP2A2 or miR-382-5p on cell proliferation, and migration in vitro, respectively. Also, dual luciferase assay was used to confirm the interactions among circAP2A2, miR-382-5p, and VEGFA.

Results: CircAP2A2 was confirmed to be highly expressed in IH. CircAP2A2 knockdown or miR-382-5p overexpression decreased the proliferation, colony formation, migration, and invasion of HemECs and HUVEC cells.

Conclusion: CircAP2A2 could promote proliferation and invasion of IH by regulating miR-382-5p/VEGFA axis.

Keywords
VEGFA; circAP2A2; circRNA; infantile hemangiomas; miR-382-5p.
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