2. Academic Validation
  3. Discovery of 3H-pyrrolo[2,3-c]quinolines with activity against Mycobacterium tuberculosis by allosteric inhibition of the glutamate-5-kinase enzyme

Discovery of 3H-pyrrolo[2,3-c]quinolines with activity against Mycobacterium tuberculosis by allosteric inhibition of the glutamate-5-kinase enzyme

  • Eur J Med Chem. 2022 Mar 15;232:114206. doi: 10.1016/j.ejmech.2022.114206.
Michele Panciera 1,  Emilio Lence 2,  Ángela Rodríguez 2,  Begoña Gracia 3,  José A Aínsa 4,  Clara Marco-Marín 5,  Vicente Rubio 5,  Carlos Roque Duarte Correia 6,  Concepción González-Bello 7
Affiliations

Affiliations

  • 1 Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, Jenaro de la Fuente s/n, 15782, Santiago de Compostela, Spain; Institute of Chemistry, State University of Campinas, Josue de Castro St, Campinas, São Paulo, 13083-970, Brazil.
  • 2 Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, Jenaro de la Fuente s/n, 15782, Santiago de Compostela, Spain.
  • 3 Departamento de Microbiología, Pediatría, Radiología y Salud Pública, Facultad de Medicina and BIFI, Universidad de Zaragoza, Domingo Miral s/n, 50009, Zaragoza, Spain; Instituto de Investigación Sanitaria Aragón (IIS-Aragón), Av. San Juan Bosco 13, 50009, Zaragoza, Spain; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Av. Monforte de Lemos 3-5, 28029, Madrid, Spain.
  • 4 Departamento de Microbiología, Pediatría, Radiología y Salud Pública, Facultad de Medicina and BIFI, Universidad de Zaragoza, Domingo Miral s/n, 50009, Zaragoza, Spain.
  • 5 Instituto de Biomedicina de Valencia (IBV-CSIC), CIBER de Enfermedades Raras (CIBERER-ISCIII), Valencia, Spain.
  • 6 Institute of Chemistry, State University of Campinas, Josue de Castro St, Campinas, São Paulo, 13083-970, Brazil.
  • 7 Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, Jenaro de la Fuente s/n, 15782, Santiago de Compostela, Spain. Electronic address: [email protected].
PMID: 35219949 DOI: 10.1016/j.ejmech.2022.114206
Abstract

The therapeutic potential of 3H-pyrrolo[2,3-c]quinolines-the main core of Marinoquinoline natural products-has been explored for the development of new anti-TB agents. The chemical modification of various positions in this scaffold has led to the discovery of two pyrroloquinolines (compounds 50 and 54) with good in vitro activity against virulent strains of Mycobacterium tuberculosis (H37Rv, MIC = 4.1 μM and 4.2 μM, respectively). Enzymatic assays showed that both derivatives are inhibitors of glutamate-5-kinase (G5K, encoded by proB gene), an essential Enzyme for this pathogen involved in the first step of the proline biosynthesis pathway. G5K catalyzes the phosphoryl-transference of the γ-phosphate group of ATP to L-glutamate to provide L-glutamyl-5-phosphate and ADP, and also regulates the synthesis of L-proline. The results of various molecular dynamics simulation studies revealed that the inhibition of G5K would be caused by allosteric interaction of these compounds with the interface between Enzyme domains, against different pockets and with distinct recognition patterns. The binding of compound 54 promotes long-distance conformational changes at the L-glutamate binding site that would prevent it from anchoring for catalysis, while compound 50 alters the ATP binding site architecture for recognition. Enzyme assays revealed that compound 50 caused a substancial increase in the Kmapp for ATP, while no significant effect was observed for derivative 54. This work also demonstrates the potential of the G5K Enzyme as a biological target for the development of new anti-TB drugs.

Keywords

Allosteric inhibition; Glutamate-5-kinase; Molecular dynamics simulation studies; Pyrroloquinoline; Target shape-motion; Tuberculosis.

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