2. Academic Validation
  3. TPM1 mediates inflammation downstream of TREM2 via the PKA/CREB signaling pathway

TPM1 mediates inflammation downstream of TREM2 via the PKA/CREB signaling pathway

  • J Neuroinflammation. 2022 Oct 14;19(1):257. doi: 10.1186/s12974-022-02619-3.
Rong Li 1 2,  Jing Zhang 3,  Qiong Wang 4,  Meng Cheng 3,  Bin Lin 5 6 7
Affiliations

Affiliations

  • 1 School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. [email protected].
  • 2 Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Shatin, Hong Kong. [email protected].
  • 3 School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
  • 4 Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Shatin, Hong Kong.
  • 5 School of Optometry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. [email protected].
  • 6 Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Shatin, Hong Kong. [email protected].
  • 7 Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, Kowloon, Hong Kong. [email protected].
PMID: 36241997 DOI: 10.1186/s12974-022-02619-3
Abstract

Background: Microglia, the innate immune cells in the central nervous system, play an essential role in brain homeostasis, neuroinflammation and brain infections. Dysregulated microglia, on the other hand, are associated with neurodegenerative diseases, yet the mechanisms underlying pro-inflammatory gene expression in microglia are incompletely understood.

Methods: We investigated the role of the actin-associated protein tropomyosin 1 (TPM1) in regulating pro-inflammatory phenotype of microglia in the retina by using a combination of Cell Culture, immunocytochemistry, Western blot, qPCR, TUNEL, RNA sequencing and electroretinogram analysis. TREM2-/- mice were used to investigate whether TPM1 regulated pro-inflammatory responses downstream of TREM2. To conditionally deplete microglia, we backcrossed CX3CR1CreER mice with Rosa26iDTR mice to generate CX3CR1CreER:Rosa26iDTR mice.

Results: We revealed a vital role for TPM1 in regulating pro-inflammatory phenotype of microglia. We found that TPM1 drove LPS-induced inflammation and neuronal death in the retina via the PKA/CREB pathway. TPM1 knockdown ameliorated LPS-induced inflammation in WT retinas yet exaggerated the inflammation in TREM2-/- retinas. RNA sequencing revealed that genes associated with M1 microglia and A1 astrocytes were significantly downregulated in LPS-treated WT retinas but upregulated in LPS-treated TREM2-/- retinas after TPM1 knockdown. Mechanistically, we demonstrated that CREB activated by TPM1 knockdown mediated anti-inflammatory genes in LPS-treated WT retinas but pro-inflammatory genes in LPS-treated TREM2-/- retinas, suggesting a novel role for TREM2 as a brake on TPM1-mediated inflammation. Furthermore, we identified that TPM1 regulated inflammation downstream of TREM2 and in a microglia-dependent manner.

Conclusions: We demonstrate that TPM1 mediates inflammation downstream of TREM2 via the PKA/CREB signaling pathway. Our findings suggest that TPM1 could be a potential target for therapeutic intervention in brain diseases.

Keywords

CREB; Inflammation; Retina; TREM2; Tropomyosin 1.

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