2. Academic Validation
  3. N-region of Cry1Ia: a novel fusion tag for Escherichia coli and Pichia pastoris

N-region of Cry1Ia: a novel fusion tag for Escherichia coli and Pichia pastoris

  • J Biotechnol. 2023 Feb 21;S0168-1656(23)00039-1. doi: 10.1016/j.jbiotec.2023.02.006.
Juanli Zhao 1,  Pu Zhou 1,  Luyao Zhang 1,  Wenhui Liu 1,  Wei Liu 1,  Yuqi Zhang 1,  Yi Li 1,  Zongyong Shi 2,  Jianhua Gao 3
Affiliations

Affiliations

  • 1 Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China.
  • 2 Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China. Electronic address: [email protected].
  • 3 Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Jinzhong, 030801, Shanxi, China. Electronic address: [email protected].
PMID: 36822476 DOI: 10.1016/j.jbiotec.2023.02.006
Abstract

Secretory signal Peptides (SPs) can increase enhanced green fluorescent protein (eGFP) expression in cytosol. In this study, SPs Iasp (Cry1Ia), Vasp (Vip3A), and their local sequences were used as fusion tags to compare their effects on eGFP expression in Escherichia coli MC4100 and Pichia pastoris GS115. In E coli, the solubility was almost opposite between the proteins encoded by Vegfp and Iegfp. This may be because the overall hydrophobicity of the SPs differed. When the hydrophobic H-region and C-region were removed, the negative effects on eGFP solubility of the N-regions of both SPs (IaN and VN) were significantly reduced without compromise on the expression level. IaN promotes eGFP protein yield 7.1-fold more than Iasp, and using this peptide in tandem (Ia3N) further enhanced fluorescent fusion protein solubility with an efficacy similar to that of a polycationic tag. Furthermore, the GS-IaNeGFP strain produced the highest fluorescent signal intensity when these fusion proteins were expressed in P. pastoris, and the expression was higher than in other strains, including eGFP. In conclusion, we revealed the potential of the N-region of Iasp as a fusion tag in both prokaryotic and eukaryotic cells and further demonstrated the value of the N-regions of abundant SPs.

Keywords

Fusion tag; N-region; Protein expression; Signal peptide; Solubility.

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