2. Academic Validation
  3. Elaboration and characterization of ε-polylysine‑sodium alginate nanoparticles for sustained antimicrobial activity

Elaboration and characterization of ε-polylysine‑sodium alginate nanoparticles for sustained antimicrobial activity

  • Int J Biol Macromol. 2023 Aug 16:251:126329. doi: 10.1016/j.ijbiomac.2023.126329.
Xiaojie Zhou 1,  Chunmin Guan 2,  Qianqian Ma 2,  Tianqing Lan 2,  Qinlu Lin 2,  Wenhua Zhou 2,  Chun Liu 3
Affiliations

Affiliations

  • 1 National Engineering Research Center for Rice and By-product Deep Processing, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China; Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, College of Food and Chemical Engineering, Shaoyang University, Shaoyang 422099, China.
  • 2 National Engineering Research Center for Rice and By-product Deep Processing, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
  • 3 National Engineering Research Center for Rice and By-product Deep Processing, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China. Electronic address: [email protected].
PMID: 37595718 DOI: 10.1016/j.ijbiomac.2023.126329
Abstract

The ε-polylysine (ε-PL) is a food-grade antimicrobial substance. The cationic ε-PL molecules may interact with anionic components of food matrix causing turbidity, sedimentation, and hampering the antimicrobial activity. Herein, sodium alginate (SA) was used as wall material to encapsulate ε-PL, thereby to synthesize ε-PL-SA nanoparticles (ε-PL-SA-NPs). Monosaccharide composition and molecular weight of SA were characterized. The synthetic scheme is optimized and physicochemical characteristics and antimicrobial potential was investigated. Findings indicate that SA primarily consisted of mannuronic acid (95.25 %), weight average molecular weight (Mw) of SA was 176.464 kDa, and the molecular configuration of SA was irregular line clusters. The encapsulation efficiency (EE) of ε-PL in ε-PL-SA-NPs made under optimum strategy (at pH 6.0, mass ratio of ε-PL to SA is 0.14, and SA concentration is 6 mg/mL) is about 99.74 %. The particle size of ε-PL-SA-NPs is ∼541.86 nm. The SEM image showed that the ε-PL-SA-NPs had a nearly spherical morphology. Zeta-potential and FTIR data reveal the interaction between ε-PL and SA was electrostatic and the hydrogen bonding. Agar diffusion assay exhibit that ε-PL-SA-NPs had antimicrobial activity against Escherichia coli and Staphylococcus aureus. The salmon preservation experiments reveal sustained antimicrobial efficacy of ε-PL-SA-NPs.

Keywords

Antimicrobial efficacy; Characterization; Nanoparticle; Sodium alginate; ε-Polylysine.

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