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  3. N-Methylpyrrolidone

N-Methylpyrrolidone  (Synonyms: NMP)

Cat. No.: HY-Y1275 Purity: 99.99%
COA Handling Instructions

N-Methylpyrrolidone (1-Methyl-2-pyrrolidinone), a five-membered cyclic amide, is an organic polar solvent. N-Methylpyrrolidone is extensively used in the manufacture of adhesives, paints, fuels, and pharmaceuticals.

For research use only. We do not sell to patients.

N-Methylpyrrolidone Chemical Structure

N-Methylpyrrolidone Chemical Structure

CAS No. : 872-50-4

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 55 In-stock
10 mM * 1 mL in DMSO USD 55 In-stock
50 g USD 50 In-stock
100 g USD 70 In-stock
> 100 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of N-Methylpyrrolidone:

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N-Methylpyrrolidone (1-Methyl-2-pyrrolidinone), a five-membered cyclic amide, is an organic polar solvent. N-Methylpyrrolidone is extensively used in the manufacture of adhesives, paints, fuels, and pharmaceuticals[1][2].

Clinical Trial
Molecular Weight







Colorless to light yellow




Room temperature in continental US; may vary elsewhere.

Pure form -20°C 3 years
4°C 2 years
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (1008.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)


Volume (start)


Concentration (final)


Volume (final)


In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:



Animal weight
(per animal)


Dosing volume
(per animal)


Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
Tween-80 +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.99%


Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 10.0878 mL 50.4388 mL 100.8776 mL 252.1941 mL
5 mM 2.0176 mL 10.0878 mL 20.1755 mL 50.4388 mL
10 mM 1.0088 mL 5.0439 mL 10.0878 mL 25.2194 mL
15 mM 0.6725 mL 3.3626 mL 6.7252 mL 16.8129 mL
20 mM 0.5044 mL 2.5219 mL 5.0439 mL 12.6097 mL
25 mM 0.4035 mL 2.0176 mL 4.0351 mL 10.0878 mL
30 mM 0.3363 mL 1.6813 mL 3.3626 mL 8.4065 mL
40 mM 0.2522 mL 1.2610 mL 2.5219 mL 6.3049 mL
50 mM 0.2018 mL 1.0088 mL 2.0176 mL 5.0439 mL
60 mM 0.1681 mL 0.8406 mL 1.6813 mL 4.2032 mL
80 mM 0.1261 mL 0.6305 mL 1.2610 mL 3.1524 mL
100 mM 0.1009 mL 0.5044 mL 1.0088 mL 2.5219 mL
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N-Methylpyrrolidone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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