2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. NO Synthase Reactive Oxygen Species
  4. Anti-inflammatory agent 38

Anti-inflammatory agent 38 (compound 23d) is a potent Nrf2/HO-1 pathway inhibitor, with an IC50 value of 0.38 μM for NO. Anti-inflammatory agent 38 can significantly reduce the level of ROS in cells. Anti-inflammatory agent 38 can be used for researching anti-inflammatory.

For research use only. We do not sell to patients.

Anti-inflammatory agent 38 Chemical Structure

Anti-inflammatory agent 38 Chemical Structure

CAS No. : 3032633-42-1

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1 mg USD 370 In-stock
5 mg USD 940 In-stock
10 mg USD 1500 In-stock
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Based on 1 publication(s) in Google Scholar

Anti-inflammatory agent 38 Related Antibodies

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Description

Anti-inflammatory agent 38 (compound 23d) is a potent Nrf2/HO-1 pathway inhibitor, with an IC50 value of 0.38 μM for NO. Anti-inflammatory agent 38 can significantly reduce the level of ROS in cells. Anti-inflammatory agent 38 can be used for researching anti-inflammatory[1].

IC50 & Target

IC50: 0.38 μM (NO)[1]
In Vitro

Anti-inflammatory agent 38 (compound 23d) (0.25, 0.5, 1 and 2 µM; 3 hours) can reduce ROS production in RAW264.7 cells stimulated with LPS (0.5 µg/mL LPS stimulates for 12 hours)[1].
Anti-inflammatory agent 38 (0.25, 0.5, 1 and 2 µM; 3 hours) significantly increases the expression of Nrf2 and HO-1 in LPS-stimulated RAW264.7 cells in a dose-dependent manner, and promoting LPS-induced Nrf2 nuclear translocation[1]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-inflammatory agent 38 Related Antibodies

Western Blot Analysis

Cell Line: RAW264.7 (0.5 µg/mL LPS stimulates for 12 hours)[1]
Concentration: 0.25, 0.5, 1 and 2 µM
Incubation Time: 3 hours
Result: Significantly increased the expression of Nrf2 and HO-1 in a dose-dependent manner.
Molecular Weight

746.82

Formula

C36H46N2O13S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=[N+]([O-])ON=C1OCCOCCOC(CCC(O[C@H]([C@]2([H])[C@@](C[C@H](C)[C@@H]2O)(O)C(/C(C)=C/[C@](C3(C)C)([H])[C@]3([H])CC4)=O)C4=C)=O)=O)(C5=CC=CC=C5)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (133.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3390 mL 6.6951 mL 13.3901 mL
5 mM 0.2678 mL 1.3390 mL 2.6780 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (6.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3390 mL 6.6951 mL 13.3901 mL 33.4753 mL
5 mM 0.2678 mL 1.3390 mL 2.6780 mL 6.6951 mL
10 mM 0.1339 mL 0.6695 mL 1.3390 mL 3.3475 mL
15 mM 0.0893 mL 0.4463 mL 0.8927 mL 2.2317 mL
20 mM 0.0670 mL 0.3348 mL 0.6695 mL 1.6738 mL
25 mM 0.0536 mL 0.2678 mL 0.5356 mL 1.3390 mL
30 mM 0.0446 mL 0.2232 mL 0.4463 mL 1.1158 mL
40 mM 0.0335 mL 0.1674 mL 0.3348 mL 0.8369 mL
50 mM 0.0268 mL 0.1339 mL 0.2678 mL 0.6695 mL
60 mM 0.0223 mL 0.1116 mL 0.2232 mL 0.5579 mL
80 mM 0.0167 mL 0.0837 mL 0.1674 mL 0.4184 mL
100 mM 0.0134 mL 0.0670 mL 0.1339 mL 0.3348 mL
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Anti-inflammatory agent 38 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-inflammatory agent 383032633-42-1NO SynthaseReactive Oxygen SpeciesNitric oxide synthasesNOSAnti-inflammatoryLPS stimulateRAW264.7Nrf2HO-1Inhibitorinhibitorinhibit

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