5HT1B Protein, Human (Cell-Free, His)

The 5HT1B protein is a G-protein-coupled receptor for serotonin, ergot alkaloids, and psychoactive substances that undergoes conformational changes upon ligand binding, modulating downstream effectors and inhibiting adenylyl cyclase activity. Inhibin inhibits G protein signaling and activates alternative pathways. 5HT1B Protein, Human (Cell-Free, His) is the recombinant human-derived 5HT1B protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of 5HT1B Protein, Human (Cell-Free, His) is 390 a.a., .

The 5HT1B protein functions as a G-protein coupled receptor for 5-hydroxytryptamine (serotonin) and serves as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs, and psychoactive substances like lysergic acid diethylamide (LSD). Upon ligand binding, 5HT1B undergoes a conformational change, initiating signaling through guanine nucleotide-binding proteins (G proteins) and modulating downstream effectors, such as adenylate cyclase, ultimately leading to the inhibition of adenylate cyclase activity. Members of the arrestin family play a role in inhibiting signaling via G proteins and activating alternative signaling pathways. This regulatory mechanism extends to the release of 5-hydroxytryptamine, dopamine, and acetylcholine in the brain, influencing neural activity, nociceptive processing, pain perception, mood, and behavior. Additionally, 5HT1B contributes to vasoconstriction in cerebral arteries, forming homodimers and heterodimers with HTR1D.

Human

E. coli Cell-free

N-10*His

P28222 (M1-S390)

3351

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.