Aminopeptidase N/APN Protein, Human (HEK293, His, solution)

Aminopeptidase N/APN Protein, Human (HEK 293, His) is a recombinant aminopeptidase N/APN protein with a His tag. APN (also known as CD13) is a Zn²⁺ dependent membrane-bound ectopeptidase that degrades preferentially proteins and peptides with a N-terminal neutral amino acid^[1].

Aminopeptidase N (APN, CD13) is a zinc-dependent ectopeptidase of the M1 family. It is a type II integral membrane protein and is located on the surface of many mammalian cells like fibroblasts, epithelial and myeloid cells. APN consists of 967 amino acids (aa), which can be divided into three regions. A short N-terminal region is located in the cytoplasm (aa 1–9), followed by a single-helix transmembrane domain (aa 10–27) and a large extracellular region (aa 28–967). APN is involved in multiple processes. It is most widely known for its protease activity in the renin-angiotensin system, where it proteolytically converts angiotensin III to IV. In addition to its enzymatic activity, it functions as a receptor for coronaviruses and has been proposed to participate in the endocytosis of cholesterol. Some of the functions of APN are mediated by protein-protein interactions^[2].

Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is 9172.6  pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P15144 (K69-K967)

290  [NCBI]

KAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK

110-130 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4.

NA

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Wickström M, et al. Aminopeptidase N (CD13) as a target for cancer chemotherapy. Cancer Sci. 2011;102(3):501-508.  [Content Brief]

  [2]. Kiehstaller S, et al. MMP activation-associated aminopeptidase N reveals a bivalent 14-3-3 binding motif. J Biol Chem. 2020;295(52):18266-18275.  [Content Brief]