BLK Protein, Human (His)

The BLK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signal transduction. It regulates pro-B to pre-B transition, growth arrest, and apoptosis downstream of B cell receptors by binding and phosphorylating CD79A and CD79B. BLK Protein, Human (His) is the recombinant human-derived BLK protein, expressed by E. coli , with C-6*His labeled tag. The total length of BLK Protein, Human (His) is 504 a.a., with molecular weight of ~60.0 kDa.

BLK Protein, a non-receptor tyrosine kinase, plays a vital role in B-lymphocyte development, differentiation, and signaling. Critical for B-cell receptor (BCR) signaling, BLK regulates the pro-B to pre-B transition, growth arrest, and apoptosis downstream of the B-cell receptor. It specifically binds and phosphorylates CD79A and CD79B, essential components of the BCR complex. In conjunction with FYN and LYN, BLK contributes to pre-B-cell receptor-mediated NF-kappa-B activation and indirectly stimulates BTK intramolecular autophosphorylation. Additionally, in pancreatic islets, BLK modulates beta-cell function, promoting insulin secretion in response to glucose by up-regulating PDX1 and NKX6-1. Furthermore, BLK phosphorylates CGAS, influencing its subcellular localization and function.

Human

E. coli

C-6*His

P51451 (G2-P505)

640  [NCBI]

GLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP

Approximately 60.0 kDa

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.