BLVRB Protein, Human (His)
Based on 1 Customer Validation
The BLVRB protein is a broad-specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of substrates, including flavin, biliverdin, methemoglobin, and PQQ. It is essential in heme catabolism, metabolizing linear tetrapyrrole and reducing complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. BLVRB Protein, Human (His) is the recombinant human-derived BLVRB protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The BLVRB protein is a broad-specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of substrates, including flavin, biliverdin, methemoglobin, and PQQ. It is essential in heme catabolism, metabolizing linear tetrapyrrole and reducing complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. BLVRB Protein, Human (His) is the recombinant human-derived BLVRB protein, expressed by E. coli , with N-His labeled tag.
BLVRB Protein is a broad-specificity oxidoreductase with the ability to catalyze the NADPH-dependent reduction of various flavins, including riboflavin, FAD or FMN, as well as biliverdins, methemoglobin, and PQQ (pyrroloquinoline quinone). This enzyme plays a crucial role in heme catabolism, metabolizing linear tetrapyrroles. Additionally, it can reduce complexed Fe(3+) iron to Fe(2+) when FMN and NADPH are present. In the liver, BLVRB Protein serves a significant function by converting biliverdin to bilirubin, contributing to essential metabolic processes and maintaining redox balance.
Measured by the reduction of riboflavin 5'-monophosphate (FMN) using NADPH as the cofactor. The specific activity is 733.394 pmoL/min/μg, as measured under the described conditions.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
P30043 (A2-Q206)
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Molecular Construction
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N-term
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His
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BLVRB (A2-Q206)
Accession # P30043 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
BLVRB; Green Heme-Binding Protein; Prev. FLR; GHBP; S-Nitroso-CoA-Assisted Nitrosyltransferase; FR; Biliverdin-IX Beta-Reductase; Biliverdin Reductase B (Flavin Reductase (NADPH)); NADPH-Dependent Diaphorase; Epididymis Secretory Protein Li 10; Flavin Red
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AA Sequence
AVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVVVGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ
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Molecular Weight
Approximately 25 kDa.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 10% Glycerol.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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