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BMP-2 Protein, Human/Mouse/Rat (P. pastoris, His)

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BMP-2 protein is an important member of the TGF-β superfamily and is critical in cardiogenesis, neurogenesis, and osteogenesis, inducing cartilage and bone formation. It initiates canonical BMP signaling by binding to BMPR1A and BMPR2, triggering BMPR2 phosphorylation and SMAD1/5/8 activation for gene transcription regulation. BMP-2 Protein, Human/Mouse/Rat (P. pastoris, His) is the recombinant human-derived BMP-2 protein, expressed by P. pastoris , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat; Mouse; Human
  • Source: P. pastoris
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • BMP-2 Protein, Human/Mouse/Rat (P. pastoris, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

BMP-2 protein is an important member of the TGF-β superfamily and is critical in cardiogenesis, neurogenesis, and osteogenesis, inducing cartilage and bone formation. It initiates canonical BMP signaling by binding to BMPR1A and BMPR2, triggering BMPR2 phosphorylation and SMAD1/5/8 activation for gene transcription regulation. BMP-2 Protein, Human/Mouse/Rat (P. pastoris, His) is the recombinant human-derived BMP-2 protein, expressed by P. pastoris , with N-6*His labeled tag.

Background

BMP-2 Protein, a vital member of the TGF-beta superfamily, plays essential roles in diverse developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. It induces cartilage and bone formation and initiates the canonical BMP signaling cascade by binding to type I receptor BMPR1A and type II receptor BMPR2. This complex formation triggers BMPR2 phosphorylation, activating BMPR1A, which, in turn, phosphorylates SMAD1/5/8 to modulate gene transcription. BMP-2 also engages non-canonical pathways, such as the ERK/MAP kinase signaling cascade, influencing osteoblast differentiation. Additionally, it stimulates myoblast differentiation into osteoblasts through the EIF2AK3-EIF2A-ATF4 pathway. Acting as a positive regulator of odontoblast differentiation, BMP-2 forms homodimers and interacts with various proteins, including SOSTDC1, GREM2, RGMA, RGMB, RGMC, ASPN, FBN1, FBN2, SCUBE3, TNFAIP6, and ERFE. Its intricate interactions highlight its versatile regulatory roles in multiple cellular processes.

Technical Parameters

  • Species Rat; Mouse; Human
  • Source P. pastoris
  • Tag N-6*His
  • Accession

    P12643 (Q283-R396)

  • Gene ID
    650  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • BMP-2 (Q283-R396)
      Accession # P12643
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    BMP2; SSFSC1; Prev. BMP2A; SSFSC; Bone Morphogenetic Protein 2A; BMP-2; BMP2 Protein; BDA2; Bone Morphogenetic Protein 2; BMP-2A

  • AA Sequence

    QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

  • Predicted Molecular Mass

    14.9 kDa

  • Molecular Weight

    Approximately 17 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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