CD4 Protein, Rat (HEK293, His)

CD4 Protein is expressed on the surface of helper T cells. It plays a key role in immune responses by binding to major histocompatibility complex class II molecules on antigen-presenting cells, facilitating T cell activation and cytokine production. CD4 Protein is also involved in immune regulation and tolerance. Understanding its functions can aid in developing therapies for autoimmune diseases and immunodeficiencies. CD4 Protein, Rat (HEK293, His) is the recombinant rat-derived CD4 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD4 Protein, Rat (HEK293, His) is 367 a.a., with molecular weight of ~42.5-52 kDa.

CD4, an integral membrane glycoprotein, plays a crucial role in orchestrating immune responses against external and internal threats. Primarily functioning as a coreceptor for MHC class II molecule:peptide complexes in T-cells, CD4 facilitates the recognition of antigens derived from extracellular proteins. Interacting simultaneously with the T-cell receptor (TCR) and MHC class II on antigen-presenting cells (APCs), CD4 recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling pathways that culminate in lymphokine production, cellular motility, adhesion, and activation of T-helper cells. Beyond T-cells, CD4 contributes to differentiation, activation, cytokine expression, and cell migration in macrophages or NK cells through a TCR/LCK-independent pathway. It also plays a role in the thymic development of T-helper cells and triggers the differentiation of monocytes into functional mature macrophages. The protein forms disulfide-linked homodimers on the cell surface and interacts with LCK, PTK2/FAK1, P4HB/PDI, and IL16. Additionally, CD4 binds to MHCII alpha and beta chains, enhancing the affinity of TCR for peptide-MHCII and facilitating adhesion between T cells and APCs through CD4 oligomerization.

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2094 μg/mL, corresponding to a specific activity is 4.776×10³ units/mg.

• 
  Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2094 μg/mL, corresponding to a specific activity is 4.776×10³ units/mg.

Rat

HEK293

C-His

P05540 (K28-T394)

24932  [NCBI]

KTVVLGKEGGSAELPCESTSRRSASFAWKSSDQKTILGYKNKLLIKGSLELYSRFDSRKNAWERGSFPLIINKLRMEDSQTYVCELENKKEEVELWVFRVTFNPGTRLLQGQSLTLILDSNPKVSDPPIECKHKSSNIVKDSKAFSTHSLRIQDSGIWNCTVTLNQKKHSFDMKLSVLGFASTSITAYKSEGESAEFSFPLNLGEESLQGELRWKAEKAPSSQSWITFSLKNQKVSVQKSTSNPKFQLSETLPLTLQIPQVSLQFAGSGNLTLTLDRGILYQEVNLVVMKVTQPDSNTLTCEVMGPTSPKMRLILKQENQEARVSRQEKVIQVQAPEAGVWQCLLSEGEEVKMDSKIQVLSKGLNQT

Approximately 42.5-52 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.