DAPK1 Protein, Human (Active, sf9, His-GST)

The DAPK1 protein is a calcium/calmodulin-dependent kinase involved in multiple cellular pathways, regulating cell survival, apoptosis, and autophagy. The DAPK1 protein also acts as a signal amplifier for NMDA receptors, causing brain damage in stroke. DAPK1 Protein, Human (sf9, His-GST) is the recombinant human-derived DAPK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

DAPK1 protein, a calcium/calmodulin-dependent serine/threonine kinase, intricately participates in multiple cellular signaling pathways, regulating cell survival, apoptosis, and autophagy. Its versatile role includes orchestrating both type I apoptotic and type II autophagic cell death signals, the former being caspase-dependent and the latter characterized by the accumulation of autophagic vesicles and being caspase-independent. DAPK1 engages in phosphorylation events with diverse substrates: it inhibits the catalytic activity, nuclear localization, and cellular function of PIN1, enhances stress fiber formation in endothelial cells by phosphorylating TPM1, and significantly decreases the binding of STX1A to STXBP1. Additionally, it phosphorylates PRKD1 to regulate JNK signaling under oxidative stress and modulates autophagy by phosphorylating BECN1, disrupting its interaction with BCL2 and BCL2L1. DAPK1 also phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. In the context of extrasynaptic NMDA receptors, DAPK1 acts as a signaling amplifier, mediating brain damage in stroke by inducing injurious Ca(2+) influx through NMDA receptor channels. Furthermore, DAPK1 collaborates with DAPK3 to phosphorylate RPL13A upon interferon-gamma activation, leading to transcript-selective translation inhibition. Notably, isoform 2 of DAPK1 lacks the ability to induce apoptosis but retains the capacity to induce membrane blebbing.

The specific activity was determined to be ≥ 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.

Human

Sf9 insect cells

N-GST;N-10*His;N-His

P53355-1 (M1-L363)

1612  [NCBI]

Partial

MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHL

Approximately 64 kDa

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.