ESRP1/RBM35A Protein, Human (sf9, His)

The ESRP1/RBM35A protein is an important mRNA splicing factor, particularly affecting epithelial cell-specific isoforms such as FGFR2-IIIb. During epithelial-mesenchymal transition (EMT), its regulatory influence extends to transcripts including CD44, CTNND1, and ENAH. ESRP1/RBM35A Protein, Human (sf9, His) is the recombinant human-derived ESRP1/RBM35A protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ESRP1/RBM35A Protein, Human (sf9, His) is 681 a.a., with molecular weight of ~77.9 KDa.

The ESRP1/RBM35A protein operates as a crucial mRNA splicing factor, intricately regulating the generation of epithelial cell-specific isoforms. Its targeted influence is notably evident in the regulation of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Additionally, ESRP1/RBM35A extends its regulatory reach to key transcripts—CD44, CTNND1, and ENAH—undergoing splicing alterations during the epithelial-to-mesenchymal transition (EMT). Functioning through direct binding to specific mRNA sequences, it specifically targets GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region within the FGFR2 mRNA. This nuanced control over splicing extends beyond epithelial-mesenchymal transitions, as ESRP1/RBM35A also governs the splicing and expression of genes pivotal to inner ear development, auditory hair cell differentiation, and cell fate specification within the cochlear epithelium.

Human

Sf9 insect cells

N-His

Q6NXG1 (M1-I681)

54845  [NCBI]

Approximately 77.9 kDa.

Greater than 80% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.