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  5. Glypican-3/GPC3 Protein, Cynomolgus (HEK293, His)

Glypican-3/GPC3 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P78304
Handling Instructions

GPC3 is a member of the heparan sulfate proteoglycans (HSPGs), and attaches to the cell surface by a glycosylphosphatidylinositol anchor. GPC3 is overexpressed in hepatocellular carcinoma (HCC) and can be used as a tumor marker for HCC. Membrane GPC3 can interact with several growth factors to promote the binding of growth factors to their receptors. GPC3 can form a complex with Wnt and activates Wnt signaling, leading to the HCC growth. In addition, GPC3 is also involved in the pathogenesis of Simpson-Golabi-Behmel overgrowth syndrome, regulates cell growth, regulates embryonic and fetal development. Glypican-3/GPC3 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Glypican-3/GPC3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Glypican-3/GPC3 Protein, Cynomolgus (HEK293, His) is 535 a.a., with molecular weight of ~42 kDa & 70-135 kDa, respectively.

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Description

GPC3 is a member of the heparan sulfate proteoglycans (HSPGs), and attaches to the cell surface by a glycosylphosphatidylinositol anchor. GPC3 is overexpressed in hepatocellular carcinoma (HCC) and can be used as a tumor marker for HCC. Membrane GPC3 can interact with several growth factors to promote the binding of growth factors to their receptors. GPC3 can form a complex with Wnt and activates Wnt signaling, leading to the HCC growth. In addition, GPC3 is also involved in the pathogenesis of Simpson-Golabi-Behmel overgrowth syndrome, regulates cell growth, regulates embryonic and fetal development[1][2][3]. Glypican-3/GPC3 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Glypican-3/GPC3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Glypican-3/GPC3 Protein, Cynomolgus (HEK293, His) is 535 a.a., with molecular weight of ~42 kDa & 70-135 kDa, respectively.
Background

GPC3 is a member of the heparan sulfate proteoglycans (HSPGs), and attaches to the cell surface by a glycosylphosphatidylinositol anchor. GPC3 is overexpressed in hepatocellular carcinoma (HCC) and can be used as a tumor marker for HCC. GPC3 plays a role in liver carcinogenesis[1]. Specifically, Membrane GPC3 can interact with several growth factors (eg: Wnt, Hedgehogs, bone morphogenetic factors, and FGF) to promote the binding of growth factors to their receptors. GPC3 can form a complex with Wnt and activates Wnt signaling, leading to the HCC growth[2].
In addition, GPC3 can regulate cell growth. GPC3 is highly expressed in mesodermal embryonic tissues, and the deletion of the GPC3 gene is involved in the pathogenesis of Simpson-Golabi-Behmel overgrowth syndrome. The interaction of GPC3 with IGF2 can reduce IGF2-mediated growth in vivo, which indicates that GPC3 negatively regulates embryonic and fetal development. Besides, GPC3 is a negative transcriptional regulator and tumor suppressor that inhibits the growth of breast, ovary, and lung cancer cells[2][3].
GPC3 has a cleavage site between Arg358 and Ser359, and can be cleaved by Furin protease[2].
Species

Cynomolgus
Source

HEK293
Tag

C-His
Accession

XP_005594665 (Q25-H559)
Gene ID

102137748  [NCBI]
Molecular Construction
N-term
GPC3 (Q25-H559)
Accession # XP_005594665
His
C-term
Synonyms
GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Molecular Weight

Approximately 42 kDa & 70-135 kDa
Purity

Greater than 95% as determined by Tris-Bis PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Glypican-3/GPC3 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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