2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. HGF & Receptors
  5. HGF
  6. GMP HGF Protein, Human (HEK293, His)

GMP HGF Protein, Human (HEK293, His)

Cat. No.: HY-P70627G
Handling Instructions

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and exhibits hepatotrophic properties. It acts as a growth factor for diverse tissues, functioning as an activating ligand for MET receptor tyrosine kinase. Upon binding, HGF promotes MET dimerization, activating the MAPK signaling cascade via TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains linked by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. GMP HGF Protein, Human (HEK293, His) is the recombinant human-derived HGF protein, expressed by HEK293, with C-6*His labeled tag. The total length of GMP HGF Protein, Human (HEK293, His) is 697 a.a., with molecular weight of 32-38 & 50-65 kDa, respectively.

For research use only. We do not sell to patients.

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Description

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and exhibits hepatotrophic properties. It acts as a growth factor for diverse tissues, functioning as an activating ligand for MET receptor tyrosine kinase. Upon binding, HGF promotes MET dimerization, activating the MAPK signaling cascade via TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains linked by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. GMP HGF Protein, Human (HEK293, His) is the recombinant human-derived HGF protein, expressed by HEK293, with C-6*His labeled tag. The total length of GMP HGF Protein, Human (HEK293, His) is 697 a.a., with molecular weight of 32-38 & 50-65 kDa, respectively.
Background

HGF protein is a potent mitogen that stimulates the growth of mature parenchymal hepatocyte cells and exhibits hepatotrophic properties. It also acts as a growth factor for various tissues and cell types. HGF protein functions as an activating ligand for the receptor tyrosine kinase MET, promoting its dimerization upon binding. This, in turn, activates MAPK signaling cascade after TMPRSS13 cleavage and activation. Structurally, HGF protein consists of an alpha chain and a beta chain that are linked together by a disulfide bond. Additionally, HGF protein interacts with SRPX2, leading to increased mitogenic activity.
Species

Human
Source

HEK293
Tag

C-6*His
Accession

P14210(Q32-S728)
Gene ID

3082
Molecular Construction
N-term
HGF (Q32-S728)
Accession # P14210
6*His
C-term
Synonyms
Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A
AA Sequence

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

Molecular Weight

32-38 &50-65 KDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<10 EU/mg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

GMP HGF Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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