GSK-3 beta Protein, Human (Active, sf9, His)

GSK-3β is a serine-threonine kinase and negative regulator of glucose homeostasis. GSK-3β has been implicated in neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. GSK-3β is widely expressed in multiple tissues, with particularly high levels in the brain and thyroid gland. GSK-3 beta Protein, Human (sf9, His) is the recombinant human-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-His labeled tag.

GSK-3 beta protein is a serine-threonine kinase in the glycogen synthase kinase subfamily that acts as a negative regulator of glucose homeostasis and plays a crucial role in energy metabolism, inflammation, endoplasmic reticulum stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene are associated with Parkinson's and Alzheimer's diseases and play an important role in neurodegenerative diseases. GSK-3 beta is widely expressed in the brain (RPKM 13.9), thyroid (RPKM 9.9), and 25 other tissues.

1. The specific activity was determined to be > 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.
2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC₅₀ of biotinylated human HG3C-CTNNB1is 0.15-0.35 μg/ml.

Human

Sf9 insect cells

N-His;N-10*His

P49841-2/NP_002084.2 (M1-T433)

2932  [NCBI]

Full Length of Isoform-2

MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKDSSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST

44-48 kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Solution.

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.5 mM TCEP, pH 7.5, 25% glycerol, 0.5 mM EDTA, 0.5 mM PMSF.
2.Supplied as a 0.22 μm filtered solution of 20 mM PB, 500 mM NaCl, 10% glycerol, 0.4 mM PMSF, 5 mM GSH.
3.Supplied as a 0.22 μm filtered solution of 20 mM PB, 500 mM NaCl, 10% glycerol, pH 7.0, 0.4 mM PMSF, 5 mM GSH, 1 mM GSSG.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.