GSK-3 beta Protein, Human (Active, sf9, His)
Based on 1 Customer Validation
GSK-3β is a serine-threonine kinase and negative regulator of glucose homeostasis. GSK-3β has been implicated in neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. GSK-3β is widely expressed in multiple tissues, with particularly high levels in the brain and thyroid gland. GSK-3 beta Protein, Human (sf9, His) is the recombinant human-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GSK-3β is a serine-threonine kinase and negative regulator of glucose homeostasis. GSK-3β has been implicated in neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. GSK-3β is widely expressed in multiple tissues, with particularly high levels in the brain and thyroid gland. GSK-3 beta Protein, Human (sf9, His) is the recombinant human-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
GSK-3 beta protein is a serine-threonine kinase in the glycogen synthase kinase subfamily that acts as a negative regulator of glucose homeostasis and plays a crucial role in energy metabolism, inflammation, endoplasmic reticulum stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene are associated with Parkinson's and Alzheimer's diseases and play an important role in neurodegenerative diseases. GSK-3 beta is widely expressed in the brain (RPKM 13.9), thyroid (RPKM 9.9), and 25 other tissues.
Verified Bioactivity
1. The specific activity was determined to be > 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.
2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1is 0.15-0.35 μg/ml.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-10*His
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Accession
P49841-2/NP_002084.2 (M1-T433)
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Molecular Construction
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N-term
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10*His
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GSK-3 beta (M1-T433)
Accession # P49841-2/NP_002084.2 -
C-term
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Protein Length
Full Length of Isoform-2
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Synonyms
GSK3B; Serine/Threonine-Protein Kinase GSK3B; Glycogen Synthase Kinase 3 Beta; GSK-3 Beta; Glycogen Synthase Kinase-3 Beta
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AA Sequence
MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKDSSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST
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Predicted Molecular Mass
50.4 kDa
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Molecular Weight
Approximately 44-53 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
1.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.5 mM TCEP, pH 7.5, 25% glycerol, 0.5 mM EDTA, 0.5 mM PMSF.
2.Supplied as a 0.22 μm filtered solution of 20 mM PB, 500 mM NaCl, 10% glycerol, 0.4 mM PMSF, 5 mM GSH.
3.Supplied as a 0.22 μm filtered solution of 20 mM PB, 500 mM NaCl, 10% glycerol, pH 7.0, 0.4 mM PMSF, 5 mM GSH, 1 mM GSSG.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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