GSK-3 beta Protein, Human (sf9, His)

The TrkA protein is a receptor protein that binds to the neurotrophin nerve growth factor (NGF). It plays a crucial role in the development and survival of neurons. GSK-3 beta Protein, Human (sf9, His) is the recombinant human-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of GSK-3 beta Protein, Human (sf9, His) is 433 a.a., with molecular weight of 44-48 kDa.

The GSK-3 beta Protein, a serine-threonine kinase within the glycogen synthase kinase subfamily, functions as a negative regulator of glucose homeostasis and plays a crucial role in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been linked to Parkinson's disease and Alzheimer's disease, highlighting its significance in neurodegenerative conditions. Ubiquitously expressed, GSK-3 beta exhibits elevated levels in the brain (RPKM 13.9), thyroid (RPKM 9.9), and 25 other tissues, underscoring its broad involvement in various physiological processes across multiple organs.

1. The specific activity was determined to be > 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.
2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC₅₀ of biotinylated human HG3C-CTNNB1is 0.15-0.35 μg/ml.

Human

Sf9 insect cells

N-His

NP_002084.2 (M1-T433)

2932  [NCBI]

MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKDSSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST

44-48 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 25% glycerol, 0.5 mM PMSF, 0.5 mM EDTA.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.