HDAC8 Protein, Human (sf9, GST)

The HDAC8 protein is a histone deacetylase that has been shown to be critical for lysine deacetylation on core histones (H2A, H2B, H3, H4). This enzymatic activity initiates epigenetic repression that affects transcription, cell cycle, and developmental events. HDAC8 Protein, Human (sf9, GST) is the recombinant human-derived HDAC8 protein, expressed by Sf9 insect cells , with C-GST labeled tag. The total length of HDAC8 Protein, Human (sf9, GST) is 377 a.a., with molecular weight of ~68 kDa.

HDAC8 Protein, a histone deacetylase, catalyzes the deacetylation of lysine residues located on the N-terminal region of core histones, namely H2A, H2B, H3, and H4, as supported by various studies. This enzymatic activity is crucial in initiating epigenetic repression, playing pivotal roles in transcriptional regulation, cell cycle progression, and developmental events. Acting within large multiprotein complexes, histone deacetylases like HDAC8 contribute to the intricate regulation of chromatin structure and function. Moreover, HDAC8 is implicated in the deacetylation of the cohesin complex protein SMC3, influencing the release of cohesin complexes from chromatin. Notably, HDAC8 may also play a role in smooth muscle cell contractility. Expanding its enzymatic repertoire, HDAC8 exhibits protein-lysine deacylase activity, specifically functioning as a protein decrotonylase by mediating the decrotonylation of histones, particularly crotonylation at lysine residues ((2E)-butenoyl).

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-GST

Q9BY41 (M1-V377)

55869  [NCBI]

Approximately 68 kDa

Greater than 88% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.