1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. HGF & Receptors
  4. HGF
  5. HGF Protein, Human (CHO)

HGF Protein, Human (CHO)

Cat. No.: HY-P7121
COA Handling Instructions

HGF Protein, Human (CHO) is a cytokine binding to Met tyrosine kinase receptor, and exhibits biological activities.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
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2 μg $55 In-stock
10 μg $150 In-stock
50 μg $490 In-stock
100 μg $830 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References


HGF Protein, Human (CHO) is a cytokine binding to Met tyrosine kinase receptor, and exhibits biological activities.


Hepatocyte growth factor (HGF) exhibits biological activities through the Met tyrosine kinase receptor. HGF plays a role in the regeneration and protection from injury and pathology in various tissues. Genetic disruption of Met in renal cells demonstrates that the HGF-Met pathway plays a role in the proliferation, morphogenesis, and survival of renal cells[1].

Biological Activity

1.The ED50 is <10 ng/mL as measured by 4MBr5 cells, corresponding to a specific activity of >1 × 105 units/mg.
2.Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 5.34 ng/mL, corresponding to a specific activity is 1.87×105 U/mg.

  • Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 5.34 ng/ml, corresponding to a specific activity is 1.87×105 U/mg.





Tag Free


P14210 (Q32-S728)

Gene ID
Molecular Construction
HGF (Q32-S728)
Accession # P14210
rHuHGF; Hepatopoietin-A; SF
AA Sequence


Molecular Weight

Single chain:88-90kDa
Alpha chain:59-61kDa
Beta chain:30-34kDa

  • Greater than 95% as determined by reducing SDS-PAGE. Human HGF is initially produced as a single, inactive polypeptide and is subsequently cleaved by serine proteases to generate a 69-kDa α chain and a 34-kDa β chain. The free α and β chains then combine to form an active heterodimer through the formation of disulfide bonds between cysteine residues. Under reducing conditions (SDS-PAGE), the protein exhibits apparent molecular masses of 90, 60, and 34 kDa, which correspond to the heavy α chain and the light β chain, respectively, due to glycosylation.

Lyophilized powder


Lyophilized after extensive dialysis against PBS. Or lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.


It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.


Room temperature in continental US; may vary elsewhere.


HGF Protein, Human (CHO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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