HTR2B Protein, Human (Cell-Free, His, SUMO)

The HTR2B protein is a multifunctional G protein-coupled receptor for serotonin and ergot alkaloids that undergoes conformational changes upon ligand binding, initiating signaling through G proteins and β-arrestin. This cascade involves the phosphatidylinositol-calcium second messenger system, regulating phosphatidylinositol 3-kinase activity and Ca(2+) release. HTR2B Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived HTR2B protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of HTR2B Protein, Human (Cell-Free, His, SUMO) is 481 a.a., with molecular weight of 70.3 kDa.

The HTR2B protein functions as a versatile G-protein coupled receptor for 5-hydroxytryptamine (serotonin) and various ergot alkaloid derivatives, as well as psychoactive substances. Upon ligand binding, HTR2B undergoes a conformational change that initiates signaling through guanine nucleotide-binding proteins (G proteins), influencing downstream effectors. Concurrently, members of the beta-arrestin family inhibit G protein signaling and activate alternative pathways. This intricate signaling cascade further involves a phosphatidylinositol-calcium second messenger system, modulating phosphatidylinositol 3-kinase activity, downstream signaling cascades, and promoting the release of Ca(2+) ions from intracellular stores. HTR2B plays a vital role in regulating dopamine and 5-hydroxytryptamine release, uptake, and extracellular levels, thereby affecting neural activity and potentially contributing to pain perception. Its involvement extends to the regulation of behavior, including impulsive behavior. Furthermore, HTR2B is indispensable for normal embryonic cardiac myocyte proliferation, heart development, protection against cardiomyocyte apoptosis, adaptation of pulmonary arteries to chronic hypoxia, vasoconstriction, normal osteoblast function and proliferation, and maintenance of bone density. It also plays a crucial role in the proliferation of interstitial cells of Cajal in the intestine, with documented interaction via its C-terminus with MPDZ.

Human

E. coli Cell-free

N-6*His;N-SUMO

P41595 (M1-V481)

3357

MALSYRVSELQSTIPEHILQSTFVHVISSNWSGLQTESIPEEMKQIVEEQGNKLHWAALLILMVIIPTIGGNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLPLVLCPAWLFLDVLFSTASIMHLCAISVDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIETDVDNPNNITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYLVKNKPPQRLTWLTVSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGDETLMRRTSTIGKKSVQTISNEQRASKVLGIVFFLFLLMWCPFFITNITLVLCDSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDAFGRYITCNYRATKSVKTLRKRSSKIYFRNPMAENSKFFKKHGIRNGINPAMYQSPMRLRSSTIQSSSIILLDTLLLTENEGDKTEEQVSYV

70.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.