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  5. Insulin Protein, Human (P.pastoris)

Insulin Protein, Human (P.pastoris)

Cat. No.: HY-P73243
COA Handling Instructions

Insulin, a crucial hormone for glucose regulation, reduces blood glucose concentration by increasing cellular permeability to monosaccharides, amino acids, and fatty acids. Its diverse actions encompass accelerating glycolysis, promoting the pentose phosphate cycle, and facilitating liver glycogen synthesis. Composed of A and B chains connected by disulfide bonds, recent studies highlight insulin's structural features essential for orchestrating metabolic processes and maintaining glucose homeostasis. Insulin Protein, Human (P.pastoris) is a recombinant protein dimer complex containing human-derived Insulin protein, expressed by P. pastoris, with tag free. Insulin Protein, Human (P.pastoris), has molecular weight of ~6 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Insulin, a crucial hormone for glucose regulation, reduces blood glucose concentration by increasing cellular permeability to monosaccharides, amino acids, and fatty acids. Its diverse actions encompass accelerating glycolysis, promoting the pentose phosphate cycle, and facilitating liver glycogen synthesis. Composed of A and B chains connected by disulfide bonds, recent studies highlight insulin's structural features essential for orchestrating metabolic processes and maintaining glucose homeostasis. Insulin Protein, Human (P.pastoris) is a recombinant protein dimer complex containing human-derived Insulin protein, expressed by P. pastoris, with tag free. Insulin Protein, Human (P.pastoris), has molecular weight of ~6 kDa.

Background

Insulin, a pivotal hormone in glucose regulation, effectively reduces blood glucose concentration by enhancing cellular permeability to monosaccharides, amino acids, and fatty acids. Its multifaceted actions include the acceleration of glycolysis, promotion of the pentose phosphate cycle, and facilitation of glycogen synthesis in the liver. The insulin molecule itself is composed of two subunits, a B chain and an A chain, intricately linked by two disulfide bonds. These structural features, as documented in recent studies, underpin the hormone's essential role in orchestrating metabolic processes critical for maintaining glucose homeostasis.

Biological Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells and the ED50 is typically 0.2-1 µg/mL.

Species

Human

Source

P. pastoris

Tag

Tag Free

Accession

P01308 (F25-K53&G90-N110)

Gene ID
Molecular Construction
N-term
Insulin (F25-K53&G90-N110)
Accession # P01308
C-term
Synonyms
Insulin; INS; IDDM; ILPR; IRDN; MODY10
Molecular Weight

Approximately 6 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Insulin Protein, Human (P.pastoris) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Insulin Protein, Human (P.pastoris)
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HY-P73243
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