Insulin Protein, Human (P.pastoris)

Insulin is an important hormone in glucose regulation, reducing blood glucose concentrations by increasing cellular permeability to simple sugars, amino acids, and fatty acids. Its multiple effects include accelerating glycolysis, promoting pentose phosphate cycle, and promoting hepatic glycogen synthesis. Insulin Protein, Human (P.pastoris) is the recombinant human-derived Insulin protein, expressed by P. pastoris, with tag free.

Insulin, a pivotal hormone in glucose regulation, effectively reduces blood glucose concentration by enhancing cellular permeability to monosaccharides, amino acids, and fatty acids. Its multifaceted actions include the acceleration of glycolysis, promotion of the pentose phosphate cycle, and facilitation of glycogen synthesis in the liver. The insulin molecule itself is composed of two subunits, a B chain and an A chain, intricately linked by two disulfide bonds. These structural features, as documented in recent studies, underpin the hormone's essential role in orchestrating metabolic processes critical for maintaining glucose homeostasis.

Insulin Protein, Human (15 pmol/kg/min; infusion) maintains plasma insulin within the physiological range in DIO mice^[5].

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells and the ED₅₀ is typically 0.2-1 µg/mL.

Human

P. pastoris

Tag Free

P01308-1 (F25-K53&G90-N110)

3630  [NCBI]

Partial

Approximately 6 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

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  [2]. Niu B , et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021 May 14;12(1):2809.  [Content Brief]

  [3]. Li S , et al. FUNDC2 promotes liver tumorigenesis by inhibiting MFN1-mediated mitochondrial fusion. Nat Commun. 2022 Jun 17;13(1):3486.  [Content Brief]

  [4]. Ladisch M R, et al. Recombinant human insulin[J]. Biotechnology progress, 1992, 8(6): 469-478.

  [5]. Xu J , et al. Acute glucose-lowering and insulin-sensitizing action of FGF21 in insulin-resistant mouse models--association with liver and adipose tissue effects. Am J Physiol Endocrinol Metab. 2009 Nov;297(5):E1105-14.  [Content Brief]