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Insulin Protein, Human (P.pastoris)

30 Cited Publications
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Based on 30 publication(s) in Google Scholar

Insulin is an important hormone in glucose regulation, reducing blood glucose concentrations by increasing cellular permeability to simple sugars, amino acids, and fatty acids. Its multiple effects include accelerating glycolysis, promoting pentose phosphate cycle, and promoting hepatic glycogen synthesis. Insulin Protein, Human (P.pastoris) is the recombinant human-derived Insulin protein, expressed by P. pastoris, with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: P. pastoris
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Insulin Protein, Human (P.pastoris)
  • Insulin Protein, Human (P.pastoris) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Top Publications Citing

Use of MCE Products

Biological Activity

Description

Insulin is an important hormone in glucose regulation, reducing blood glucose concentrations by increasing cellular permeability to simple sugars, amino acids, and fatty acids. Its multiple effects include accelerating glycolysis, promoting pentose phosphate cycle, and promoting hepatic glycogen synthesis. Insulin Protein, Human (P.pastoris) is the recombinant human-derived Insulin protein, expressed by P. pastoris, with tag free.

Background

Insulin, a pivotal hormone in glucose regulation, effectively reduces blood glucose concentration by enhancing cellular permeability to monosaccharides, amino acids, and fatty acids. Its multifaceted actions include the acceleration of glycolysis, promotion of the pentose phosphate cycle, and facilitation of glycogen synthesis in the liver. The insulin molecule itself is composed of two subunits, a B chain and an A chain, intricately linked by two disulfide bonds. These structural features, as documented in recent studies, underpin the hormone's essential role in orchestrating metabolic processes critical for maintaining glucose homeostasis.

In Vivo

Insulin Protein, Human (15 pmol/kg/min; infusion) maintains plasma insulin within the physiological range in DIO mice[5].

Verified Bioactivity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells and the ED50 is typically 0.2-1 µg/mL.

Technical Parameters

  • Species Human
  • Source P. pastoris
  • Tag Tag Free
  • Accession

    P01308-1 (F25-K53&G90-N110)

  • Gene ID
    3630  [NCBI]
  • Molecular Construction
    • N-term
    • Insulin (F25-K53&G90-N110)
      Accession # P01308-1
    • C-term
  • Protein Length

    Full Length of Insulin Chain

  • Synonyms

    INS; Proinsulin; Prev. IDDM1; PNDM4; Prev. IDDM2; IDDM; Insulin-Dependent Diabetes Mellitus 2; ILPR; Preproinsulin; IRDN; Insulin; MODY10

  • Predicted Molecular Mass

    7.1 kDa

  • Molecular Weight

    Approximately 6 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Chen S, et al. Single-cell analysis reveals transcriptomic remodellings in distinct cell types that contribute to human prostate cancer progression. Nat Cell Biol. 2021 Jan;23(1):87-98. [Content Brief]

[2]. Niu B , et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021 May 14;12(1):2809. [Content Brief]

[3]. Li S , et al. FUNDC2 promotes liver tumorigenesis by inhibiting MFN1-mediated mitochondrial fusion. Nat Commun. 2022 Jun 17;13(1):3486. [Content Brief]

[4]. Ladisch M R, et al. Recombinant human insulin[J]. Biotechnology progress, 1992, 8(6): 469-478.

[5]. Xu J , et al. Acute glucose-lowering and insulin-sensitizing action of FGF21 in insulin-resistant mouse models--association with liver and adipose tissue effects. Am J Physiol Endocrinol Metab. 2009 Nov;297(5):E1105-14. [Content Brief]

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Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    Insulin Protein, Human (P.pastoris) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb 20:e2408106. [Abstract]

    Customer Validation
     DLAT oligomerization was analyzed in MDA-MB-231 cells with or without Insulin Protein treatment following a 2-hour elesclomol-Cu pulse. Insulin Protein activated AKT1 and significantly reduced insoluble lipoylated expression and oligomerization of DLAT.

  • Insulin Protein, Human (P.pastoris) Product Image
    1/16
  • Experimental Validation Results for Insulin Protein, Human (P.pastoris)
  • Insulin Protein, Human (P.pastoris) Product Image

  • Insulin Protein, Human (P.pastoris) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb 20:e2408106. [Abstract]

    DLAT oligomerization was analyzed in MDA-MB-231 cells with or without Insulin Protein treatment following a 2-hour elesclomol-Cu pulse. Insulin Protein activated AKT1 and significantly reduced insoluble lipoylated expression and oligomerization of DLAT.