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  5. NCAM-1/CD56 Protein, Rat (HEK293, C-His)

NCAM-1/CD56 Protein, Rat (HEK293, C-His)

Cat. No.: HY-P73749A
Handling Instructions

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth. Its interaction with MDK contributes to complex cellular interactions in neural development. NCAM-1/CD56 Protein, Rat (HEK293, C-His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of NCAM-1/CD56 Protein, Rat (HEK293, C-His) is 702 a.a., with molecular weight of approximately 130 kDa.

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Description

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth. Its interaction with MDK contributes to complex cellular interactions in neural development. NCAM-1/CD56 Protein, Rat (HEK293, C-His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of NCAM-1/CD56 Protein, Rat (HEK293, C-His) is 702 a.a., with molecular weight of approximately 130 kDa.

Background

NCAM-1/CD56 Protein, a cell adhesion molecule, plays a pivotal role in various neuronal processes, including neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurites. It interacts with MDK, contributing to the intricate cellular interactions in neural development. Furthermore, NCAM-1 is part of a complex involving SLC39A6, SLC39A10, and NCAM1 itself, which regulates NCAM1 phosphorylation and facilitates its integration into focal adhesion complexes during epithelial-to-mesenchymal transition. This underscores the dynamic involvement of NCAM-1 in cell adhesion and signaling events, emphasizing its importance in both neural development and cellular processes associated with epithelial-to-mesenchymal transition.

Species

Rat

Source

HEK293

Tag

C-10*His

Accession

P13596 (L20-T721)

Gene ID

24586  [NCBI]

Molecular Construction
N-term
NCAM-1 (L20-T721)
Accession # P13596
10*His
C-term
Synonyms
CD56; MSK39; NCAM-1; Neural cell adhesion molecule 1; NCAM
AA Sequence

LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

Molecular Weight

approximately 130 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NCAM-1/CD56 Protein, Rat (HEK293, C-His)
Cat. No.:
HY-P73749A
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