Oleosin L Protein, Sesamum indicum (Cell-Free, His)

The oleosin L protein plays a structural role in stabilizing liposomes during seed drying, preventing oil coalescence and affecting liposome integrity. Its potential interactions with lipid and phospholipid moieties suggest the involvement of multiple molecules. Oleosin L Protein, Sesamum indicum (Cell-Free, His) is the recombinant Oleosin L protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli Cell-free
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The oleosin L protein plays a structural role in stabilizing liposomes during seed drying, preventing oil coalescence and affecting liposome integrity. Its potential interactions with lipid and phospholipid moieties suggest the involvement of multiple molecules. Oleosin L Protein, Sesamum indicum (Cell-Free, His) is the recombinant Oleosin L protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

Background

The Oleosin L protein appears to serve a structural role in stabilizing lipid bodies during seed desiccation by preventing the coalescence of oil, highlighting its importance in seed physiology. Its potential interaction with both lipid and phospholipid moieties of lipid bodies suggests a versatile molecular engagement, possibly influencing the lipid body's integrity and function. Furthermore, Oleosin L may play a role in providing recognition signals for specific lipase anchorage, indicating its involvement in lipolysis during seedling growth. The multifaceted functions attributed to Oleosin L underscore its significance in orchestrating essential processes related to lipid metabolism and seed development.

Technical Parameters

  • Species Others
  • Source E. coli Cell-free
  • Tag N-10*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • 10*His
    • Oleosin L (M1-S145)
      Accession # Q9XHP2
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    Oleosin L; Allergen Ses i 5; Oleosin 15 kDa; Ses i 5.0101

  • AA Sequence

    MAEHYGQQQQTRAPHLQLQPRAQRVVKAATAVTAGGSLLVLSGLTLAGTVIALTIATPLLVIFSPVLVPAVITIFLLGAGFLASGGFGVAALSVLSWIYRYLTGKHPPGADQLESAKTKLASKAREMKDRAEQFSQQPVAGSQTS

  • Predicted Molecular Mass

    16.7 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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