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OPN1MW Protein, Human (Cell-Free, His)

Cat. No.: HY-P702399
Handling Instructions

OPN1MW protein, vital for vision, plays a crucial role in visual pigments, absorbing light and facilitating vision. These pigments consist of opsin, forming a covalent bond with cis-retinal. The OPN1MW protein, along with cis-retinal, contributes to the intricate molecular mechanism essential for perceiving light stimuli, enabling the visual process. OPN1MW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1MW protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPN1MW Protein, Human (Cell-Free, His) is 364 a.a., with molecular weight of 46.6 kDa.

For research use only. We do not sell to patients.

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Description

OPN1MW protein, vital for vision, plays a crucial role in visual pigments, absorbing light and facilitating vision. These pigments consist of opsin, forming a covalent bond with cis-retinal. The OPN1MW protein, along with cis-retinal, contributes to the intricate molecular mechanism essential for perceiving light stimuli, enabling the visual process. OPN1MW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1MW protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPN1MW Protein, Human (Cell-Free, His) is 364 a.a., with molecular weight of 46.6 kDa.

Background

The OPN1MW protein, integral to the process of vision, serves a crucial role as part of visual pigments, the molecules responsible for absorbing light and mediating vision. These pigments are composed of an apoprotein, known as opsin, which forms a covalent linkage with cis-retinal. Together, the OPN1MW protein and cis-retinal contribute to the intricate molecular mechanism essential for the perception of light stimuli, enabling the visual process to unfold.

Species

Human

Source

E. coli Cell-free

Tag

N-10*His

Accession

P04001 (M1-A364)

Gene ID

/

Molecular Construction
N-term
10*His
OPN1MW (M1-A364)
Accession # P04001
C-term
Synonyms
Medium-wave-sensitive opsin 1; Green cone photoreceptor pigment; Green-sensitive opsin; GOP
AA Sequence

MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVIASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISVVNQVYGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWMVVCKPFGNVRFDAKLAIVGIAFSWIWAAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCITPLSIIVLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMVLAFCFCWGPYAFFACFAAANPGYPFHPLMAALPAFFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA

Molecular Weight

46.6 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

OPN1MW Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
OPN1MW Protein, Human (Cell-Free, His)
Cat. No.:
HY-P702399
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