SYT12/Synaptotagmin-12 Protein, Rat (Cell-Free, His)

SYT12/Synaptotagmin-12 is a unique synaptic vesicle phosphoprotein that enhances spontaneous neurotransmitter release without affecting induced release. SYT12, characterized by its lack of Ca(2+) and phospholipid binding, is critical for long-term potentiation of mossy fibers, emphasizing its role in synaptic plasticity. SYT12/Synaptotagmin-12 Protein, Rat (Cell-Free, His) is the recombinant rat-derived SYT12/Synaptotagmin-12 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of SYT12/Synaptotagmin-12 Protein, Rat (Cell-Free, His) is 421 a.a., with molecular weight of 50.7 kDa.

The SYT12/Synaptotagmin-12 protein emerges as a distinctive synaptic vesicle phosphoprotein that plays a pivotal role in enhancing spontaneous neurotransmitter release while having no impact on induced neurotransmitter release. Unlike other synaptotagmins, SYT12 does not bind Ca(2+) or phospholipids, setting it apart in its functional characteristics. Notably, it proves essential for mossy-fiber long-term potentiation in the hippocampus, underscoring its specific involvement in synaptic plasticity processes. Functionally, SYT12 operates as a homodimer and can also form heterodimers, adding to its dynamic role in vesicle regulation. Moreover, it interacts with SYT1, further highlighting its intricate involvement in synaptic mechanisms and suggesting potential crosstalk between different synaptotagmin isoforms in neurotransmitter release dynamics.

Rat

E. coli Cell-free

N-6*His

P97610 (M1-N421)

191595

MAVDVTEYHLSVIKSPPGWEVGVYAAGALALLGIAAVSLWKLWTSGSFPSPSPFPNYDYRYLQQKYGEAYVEAKLKRVPPWNAQRTTTRGPPSRKGSLSIEDTFESISELGPLELMGRELDLAPYGTLRKSQSADSLNSISSVSNTFGQDFTLGQVEVSMDYDGASHTLHVAVLQGKDLLEREEATFESCFMRVSLLPDEQIVGISRIQRNAYSIFFDEKFSVPLDPTALEEKSLRFSVFGIDEDERNVSTGVVELKLSVLDLPLQPFSGWLYLQDQNKAADAVGEILLSLSYLPTAERLTVVVVKAKNLIWTNDKTTADPFVKVYLLQDGRKMSKKKTAVKRDDPNPVFNEAMIFSVPAIVLQDLSLRVTVAESSSDGRGDNVGHVIIGPGASGMGTTHWNQMLATLRRPVSMWHPVRRN

50.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.