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TARDBP Protein, Human (P.pastoris, His)

Cat. No.: HY-P71788
COA Handling Instructions

TARDBP protein is an RNA-binding factor with RRM1 and RRM2 that binds GU repeats and regulates RNA splicing in long introns and 3'UTRs.It affects proteins critical for neuronal survival and neurodegenerative diseases.TARDBP Protein, Human (P.pastoris, His) is the recombinant human-derived TARDBP protein, expressed by P.pastoris , with N-6*His labeled tag.

For research use only. We do not sell to patients.

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10 μg $210 In-stock
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Description

TARDBP protein is an RNA-binding factor with RRM1 and RRM2 that binds GU repeats and regulates RNA splicing in long introns and 3'UTRs.It affects proteins critical for neuronal survival and neurodegenerative diseases.TARDBP Protein, Human (P.pastoris, His) is the recombinant human-derived TARDBP protein, expressed by P.pastoris , with N-6*His labeled tag.

Background

The TARDBP protein, an RNA-binding factor, plays a multifaceted role in various steps of RNA biogenesis and processing. Utilizing its two RNA recognition motifs, RRM1 and RRM2, TARDBP preferentially binds GU-repeats on RNA molecules, predominantly localized within long introns and the 3'UTR of mRNAs. This binding activity regulates the splicing of numerous non-coding and protein-coding RNAs, impacting proteins crucial for neuronal survival and those relevant to neurodegenerative diseases. TARDBP also contributes to mitochondrial homeostasis by regulating the processing of mitochondrial transcripts and modulates mRNA stability by recruiting CNOT7/CAF1 deadenylase to mRNA 3'UTR, leading to poly(A) tail deadenylation. In response to oxidative insult, TARDBP associates with stalled ribosomes in stress granules, contributing to cell survival. Moreover, it participates in skeletal muscle formation and regeneration by forming cytoplasmic myo-granules and binding mRNAs encoding sarcomeric proteins. Additionally, TARDBP is involved in the maintenance of circadian clock periodicity by stabilizing CRY1 and CRY2 proteins in a FBXL3-dependent manner and negatively regulates the expression of CDK6. Interactions with various proteins, including BRDT, ATXN2, MATR3, UBQLN2, HNRNPA2B1, ZNF106, CNOT7/CAF1, CRY2, and PPIA/CYPA, highlight the diverse roles of TARDBP in RNA regulation and cellular processes.

Species

Human

Source

P. pastoris

Tag

N-6*His

Accession

Q13148 (M1-G396)

Gene ID
Molecular Construction
N-term
6*His
TARDBP (M1-G396)
Accession # Q13148
C-term
Synonyms
ALS10; TAR DNA binding protein 43; TAR DNA binding protein; TAR DNA-binding protein 43; TARDBP; TDP 43; TDP-43; TDP43
AA Sequence

MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSG

Molecular Weight

Approximately 44.9 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TARDBP Protein, Human (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TARDBP Protein, Human (P.pastoris, His)
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HY-P71788
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