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  2. Cancer Models DNA Alkylator/Crosslinker
  3. Colon Cancer Models
  4. Azoxymethane

Azoxymethane  (Synonyms: AOM)

Cat. No.: HY-111375 Purity: ≥99.0%
COA Handling Instructions

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.

For research use only. We do not sell to patients.

Azoxymethane Chemical Structure

Azoxymethane Chemical Structure

CAS No. : 25843-45-2

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5 mg (135 mM * 500 μL in Water) USD 220 In-stock
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10 mg (135 mM * 1 mL in Water) USD 415 In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

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Description

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts.

In Vitro

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts. On an equal protein basis, hepatic microsomes are much more active than SI and colon microsomes in NADPH-dependent Azoxymethane bioactivation and N7-mG adduct formation. Hepatic microsomes show the highest activity in the hydroxylation of Azoxymethane, followed by SI and colon microsomes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Azoxymethane is a colon carcinogen which leads to the formation of DNA adducts. On an equal protein basis, hepatic microsomes are much more active than SI and colon microsomes in NADPH-dependent Azoxymethane bioactivation and N7-mG adduct formation. Hepatic microsomes show the highest activity in the hydroxylation of Azoxymethane, followed by SI and colon microsomes[1].

Azoxymethane can be used in animal modeling to construct tumor models. Regardless of the strain, the amounts of O6-mG and N7-mG produced by Azoxymethane are highest in the liver, followed by proximal and distal colons, which have similar levels, and then by duodenum, jejunum and ileum. Results indicate that the Azoxymethane-induced DNA adduct formation in the SI and colon does not depend on bioactivation by hepatic P450 enzymes. Irrespective of the mouse strain, no aberrant crypt foci (ACF) is detected in the colons of saline-treated mice; in contrast, colonic ACF is detected in all three strains of Azoxymethane-treated mice[1]. The Azoxymethane-treated athymic mice have approximately an 11-fold lower tumor incidence than similarly treated WT animals[2].

Induction of Colonic
Background
Azoxymethane induces induces G to A transitions and leads to activating mutations in K-ras and β-catenin in colonocytes, ctivation of Wnt signaling by accumulation of beta-catenin is a major mechanism in the azoxymethane-induced colon carcinogenesis model.
Specific Mmodeling Methods
Mice: 20-25 g•Male A/J mice&bull
Administration: 5 mg/kg• i.p.• weekly for 6 weeks
Note
Modeling Indicators
Cellular/tissue level: Induced hyperproliferation and microadenoma formation, Increased the cyclin D1 and COX-2 expression.
Molecular changes: Increased the protein expression of pro-TGF-α, pEGFR, pErbB2, pERK, induces K-ras mutations.
Correlated Product(s)
Opposite Product(s) Gefitinib (HY-50895)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

74.08

Formula

C2H6N2O

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

C/[N+]([O-])=N/C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Purity & Documentation

Purity: ≥99.0%

References
Kinase Assay
[1]

The assay for Azoxymethane-induced in vitro DNA adduct formation is performed. Briefly, microsomes (0.5 to 2.0 mg/mL) are incubated with calf thymus DNA (1 mg/mL) and Azoxymethane (200 μM) in a total volume of 1.0 mL. The assay buffer consists of 0.1 M Tris-HCl (pH 7.4), 1 mM EDTA, 20 mM MgCl2, 0.3 M KCl, and 1.5 mM NADPH. Incubations are carried out at 37°C for 60 min in a shaking water bath. An additional 30 nM of NADPH is added after the first 30 min. The reaction is stopped by the addition of 0.5 mL of ice-cold 7.5 M ammonium acetate. DNA is then extracted for tissue homogenates. Control incubations are performed without NADPH[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Male, 8 to 10 week old, WT-A/J, IECN-A/J, and LCN-A/J mice (8 per group) are treated with either saline or Azoxymethane (7.5 mg/kg BW, s.c.), once weekly for 3 weeks. Mice are sacrificed 6 weeks post-treatment for aberrant crypt foci (ACF) detection. The entire colon is excised. A longitudinal incision is made along the entire length of the colon, which is further cut into two equal-length segments, representing proximal and distal portions of the colon. The segments are dipped in PBS to remove fecal pellets and then kept flat between filter papers in 10% buffered formalin for at least 24 h. Subsequently, the colons are immersed in freshly prepared 0.1% methylene blue for 10 min and rinsed briefly in deionized H2O to remove excess dye. The colon is mounted carefully on a microscope slide with the mucosal surface side up and viewed under a light microscope. The ACF in the entire mucosal surface of the colon are counted blindly and independently by two investigators and recorded[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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