CB 300919
Based on 1 Customer Validation
CB 300919 is a quinazoline-based antitumour agent with high activity in the CH1 human ovarian tumour xenograft. CB 300919 has a continuous exposure (96 h) growth inhibition IC50 value of 2 nM in human CH1 ovarian tumor xenograft. CB 300919 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- CAS No.: 289715-28-2
- Formula: C32H34ClN7O2
- Molecular Weight:584.11
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| WIL2 | EC50 |
0.49 nM
Compound: 2
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Cytotoxicity against human W1L2 cells
Cytotoxicity against human W1L2 cells
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[PMID: 21080724] |
| WIL2 | IC50 |
0.32 nM
Compound: 5a
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Growth inhibitory activity against human lymphoblastoid W1L2 cell line in presence of 10 uM thymidine/50 uM hypoxanthine
Growth inhibitory activity against human lymphoblastoid W1L2 cell line in presence of 10 uM thymidine/50 uM hypoxanthine
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[PMID: 12166942] |
| WIL2 | IC50 |
0.49 nM
Compound: 5a
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Growth inhibitory activity against human lymphoblastoid W1L2 cell line
Growth inhibitory activity against human lymphoblastoid W1L2 cell line
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[PMID: 12166942] |
Chemical Information
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CAS No. 289715-28-2
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Appearance Solid
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Molecular Weight 584.11
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Formula C32H34ClN7O2
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Color White to off-white
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SMILES
CN1CCN(CC1)CC2=NC3=C(C(N2C)=O)C=C(CN(CC#C)C4=CC=C(C=C4)C(NCC5=CC=CN=C5)=O)C(Cl)=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 100 mg/mL (171.20 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (5.14 mM); Clear solution
This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3 mg/mL (5.14 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7120 mL | 8.5600 mL | 17.1201 mL | 42.8002 mL |
| 5 mM | 0.3424 mL | 1.7120 mL | 3.4240 mL | 8.5600 mL | |
| 10 mM | 0.1712 mL | 0.8560 mL | 1.7120 mL | 4.2800 mL | |
| 15 mM | 0.1141 mL | 0.5707 mL | 1.1413 mL | 2.8533 mL | |
| 20 mM | 0.0856 mL | 0.4280 mL | 0.8560 mL | 2.1400 mL | |
| 25 mM | 0.0685 mL | 0.3424 mL | 0.6848 mL | 1.7120 mL | |
| 30 mM | 0.0571 mL | 0.2853 mL | 0.5707 mL | 1.4267 mL | |
| 40 mM | 0.0428 mL | 0.2140 mL | 0.4280 mL | 1.0700 mL | |
| 50 mM | 0.0342 mL | 0.1712 mL | 0.3424 mL | 0.8560 mL | |
| 60 mM | 0.0285 mL | 0.1427 mL | 0.2853 mL | 0.7133 mL | |
| 80 mM | 0.0214 mL | 0.1070 mL | 0.2140 mL | 0.5350 mL | |
| 100 mM | 0.0171 mL | 0.0856 mL | 0.1712 mL | 0.4280 mL |
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