1. Epigenetics
  2. Histone Demethylase
  3. IOX1

IOX1 

Cat. No.: HY-12304 Purity: 97.04%
Handling Instructions

IOX1, 5-Carboxy-8-hydroxyquinoline, is a potent broad‐spectrum inhibitor of 2OG oxygenases, including the JmjC demethylases. IOX1 inhibits KDM4C, KDM4E, KDM2A, KDM3A and KDM6B with IC50 values of 0.6 μM, 2.3 μM, 1.8 μM, 0.1 μM and 1.4 μM, respectively.

For research use only. We do not sell to patients.

IOX1 Chemical Structure

IOX1 Chemical Structure

CAS No. : 5852-78-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 77 In-stock
Estimated Time of Arrival: December 31
5 mg USD 70 In-stock
Estimated Time of Arrival: December 31
10 mg USD 120 In-stock
Estimated Time of Arrival: December 31
50 mg USD 480 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE IOX1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

IOX1, 5-Carboxy-8-hydroxyquinoline, is a potent broad‐spectrum inhibitor of 2OG oxygenases, including the JmjC demethylases. IOX1 inhibits KDM4C, KDM4E, KDM2A, KDM3A and KDM6B with IC50 values of 0.6 μM, 2.3 μM, 1.8 μM, 0.1 μM and 1.4 μM, respectively[1].

In Vitro

IOX1 (0-200 µM; 2 hours) inhibits the proliferation and migration of vascular smooth muscle cells (VSMCs) stimulated with angiotensin II (Ang II) in a concentration-dependent manner[2].
IOX1 (200 µM; 24 hours) blocks the cell cycle progression of angiotensin II (Ang II)-VSMCs by increasing the percentage of cells in the G0/G1 phase[2].
IOX1 (50-200 µM; 2 hours) attenuates cyclin D1 and upregulates p21 mRNA levels in a concentration-dependent[2].
IOX1 (50-200 µM; 2 hours) mediates cyclin D1 and p21 expression by regaining H3K9me3[2].

Cell Proliferation Assay[2]

Cell Line: Vascular smooth muscle cells (VSMCs)
Concentration: 50 μM, 100 μM, 200 μM
Incubation Time: Pretreated 2 hours
Result: Exhibited a decrease in proliferation and migration.

Cell Cycle Analysis[2]

Cell Line: Vascular smooth muscle cells (VSMCs)
Concentration: 200 μM
Incubation Time: 24 hours
Result: Slowed down the progression of the cell cycle from the G0/G1 to the S phase.

RT-PCR[2]

Cell Line: Vascular smooth muscle cells (VSMCs)
Concentration: 50 μM, 100 μM, 200 μM
Incubation Time: 2 hours
Result: Decreased cyclin D1 mRNA expression and increased p21 mRNA expression.

RT-PCR[2]

Cell Line: Vascular smooth muscle cells (VSMCs)
Concentration: 50 μM, 100 μM, 200 μM
Incubation Time: 2 hours
Result: Enhanced the total protein levels of H3K9me3.
Molecular Weight

189.17

Formula

C₁₀H₇NO₃

CAS No.

5852-78-8

SMILES

O=C(C1=C2C=CC=NC2=C(O)C=C1)O

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 13.33 mg/mL (70.47 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.2863 mL 26.4313 mL 52.8625 mL
5 mM 1.0573 mL 5.2863 mL 10.5725 mL
10 mM 0.5286 mL 2.6431 mL 5.2863 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (13.22 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (13.22 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
IOX1
Cat. No.:
HY-12304
Quantity: