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  3. JSH-23

JSH-23 

Cat. No.: HY-13982 Purity: 99.48%
Handling Instructions

JSH-23 is an NF-κB inhibitor which inhibits NF-κB transcriptional activity with an IC50 of 7.1 μM in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7. JSH-23 inhibits nuclear translocation of NF-κB p65 without affecting IκBα degradation.

For research use only. We do not sell to patients.

JSH-23 Chemical Structure

JSH-23 Chemical Structure

CAS No. : 749886-87-1

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 117 In-stock
Estimated Time of Arrival: December 31
5 mg USD 106 In-stock
Estimated Time of Arrival: December 31
10 mg USD 190 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 16 publication(s) in Google Scholar

Top Publications Citing Use of Products

    JSH-23 purchased from MCE. Usage Cited in: PLoS One. 2016 Nov 16;11(11):e0166740.

    HG-induced apoptosis in neonatal rat cardiomyocytes is mediated by activation of the NF-κB pathway. (A-B). Neonatal rat cardiomyocytes are pretreated with 10–20 μM JSH-23 and exposed to HG for 36 h. Levels of caspase-3 and cleaved caspase-3 and Bax/Bcl-2 ratios are determined by western blotting.

    JSH-23 purchased from MCE. Usage Cited in: Int J Clin Exp Pathol. 2017;10(6):6544-6551.

    JSH-23 is added into MDA-MB-231 cells with the dose of 0 μmol, 1 μmol, 5 μmol when the cell planking density reaches about 60%. Then samples incubated for 24 h are collected when the cell density reaches about 80% and confirmed by western blot.

    JSH-23 purchased from MCE. Usage Cited in: Cancer Lett. 2018 Aug 1;428:77-89.

    Western blots show that the NF-κB inhibitors BAY11-7082, Parthenolide, and JSH-23 cause dramatic time- and dose-dependent reductions in MGMT protein expression in LN18 and T98G glioma cells.

    JSH-23 purchased from MCE. Usage Cited in: Front Pharmacol. 2018 Jun 21;9:660.

    By Western blotting, the α-SMA and palladin proteins are determined in cells that are pretreated with NF-κB (JSH-23), Wnt/β-catenin (XAV939), EGFR (erlotinib), p38 MAPK (TAK-715), and Smad3 (SIS3) inhibitors and are followed by TWEAK stimulation.

    JSH-23 purchased from MCE. Usage Cited in: J Mol Cell Biol. 2018 Oct 15. 

    Cytoplasmic LPS-induced GSDMB and GSDMD expression are both significantly attenuated by either JSH-23 or QNZ.

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    Description

    JSH-23 is an NF-κB inhibitor which inhibits NF-κB transcriptional activity with an IC50 of 7.1 μM in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7. JSH-23 inhibits nuclear translocation of NF-κB p65 without affecting IκBα degradation[1].

    IC50 & Target[1]

    NF-κB

    7.1 μM (IC50, in RAW 264.7 cells)

    In Vitro

    JSH-2 (1-300 μM; 24 hours) at <100 μM does not show significant cytotoxic effects on the RAW 264.7 cells[1].
    Nuclear amount of NF-κB p65 is markedly increased upon exposure to LPS for 1 h. Treatment of JSH-23 (30 μM; 1 hours) to LPS- stimulated RAW 264.7 cells decreases nuclear content of NF-κB p65 in a dose-dependent manner[1].

    Cell Cytotoxicity Assay[1]

    Cell Line: Macrophages RAW 264.7
    Concentration: 1, 3, 10, 30, 100, 300 μM
    Incubation Time: 24 hours
    Result: Did not show significant cytotoxic effects at <100 μM.

    Western Blot Analysis[1]

    Cell Line: Macrophages RAW 264.7 with LPS-stimulated
    Concentration: 30 μM
    Incubation Time: 1 hour
    Result: Decreased nuclear content of NF-κB p65 in a dose-dependent manner, corresponding to 49±4% inhibition at 3 μM, 75±7% at 10 μM and 95±8% at 30 μM.
    In Vivo

    JSH-23 (1 mg/kg, 3 mg/kg; orally administered; daily; for 2 weeks) significantly reverses the nerve conduction and nerve blood flow deficits seen in diabetic rats[1].

    Animal Model: Male Sprague Dawley diabetic rats (250-270 g)[1]
    Dosage: 1 mg/kg, 3 mg/kg
    Administration: Orally administered; daily; for 2 weeks
    Result: Produced significant improvement in motor nerve conduction velocity (MNCV).
    Molecular Weight

    240.34

    Formula

    C₁₆H₂₀N₂

    CAS No.

    749886-87-1

    SMILES

    NC1=CC(C)=CC=C1NCCCC2=CC=CC=C2

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 56 mg/mL (233.00 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.1608 mL 20.8039 mL 41.6077 mL
    5 mM 0.8322 mL 4.1608 mL 8.3215 mL
    10 mM 0.4161 mL 2.0804 mL 4.1608 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (10.40 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (10.40 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Product Name:
    JSH-23
    Cat. No.:
    HY-13982
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