1. Epigenetics
    Apoptosis
  2. Epigenetic Reader Domain
    Apoptosis
  3. MI-3

MI-3 (Synonyms: Menin-MLL inhibitor 3)

Cat. No.: HY-15223 Purity: 99.73%
Handling Instructions

MI-3 (Menin-MLL inhibitor 3) is a potent and high affinity menin-MLL inhibitor with an IC50 of 648 nM and a Kd of 201 nM.

For research use only. We do not sell to patients.

MI-3 Chemical Structure

MI-3 Chemical Structure

CAS No. : 1271738-59-0

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 172 In-stock
Estimated Time of Arrival: December 31
5 mg USD 156 In-stock
Estimated Time of Arrival: December 31
10 mg USD 252 In-stock
Estimated Time of Arrival: December 31
50 mg USD 840 In-stock
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100 mg USD 1080 In-stock
Estimated Time of Arrival: December 31
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Description

MI-3 (Menin-MLL inhibitor 3) is a potent and high affinity menin-MLL inhibitor with an IC50 of 648 nM and a Kd of 201 nM[1].

IC50 & Target

IC50: 648 nM (menin-MLL); Kd: 201 nM (menin-MLL)[1]

In Vitro

MI-3 (12.5-50 μM; HEK293 cells) treatment effectively inhibits the menin-MLL-AF9 interaction in human cells[1].
MI-3 (0-1.6 μM; 72 hours; KOPN-8 and MV4;11 cells) treatment shows an effective and dose-dependent growth inhibition in KOPN-8, MV4 and ME-1 cells[1].
MI-3 (12.5-50 μM; 48 hours; MV4;11 cells) treatment results in a substantial, and dose-dependent increase in Annexin V and AnnexinV/propidium iodide (PI) cells, demonstrating an increase in the number of cells undergoing apoptosis[1].
MI-3 (6.25-25 μM; 6 days; THP-1 cells) treatment results in substantially reduced expression of HOXA9 and MEIS1[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HEK293 cells
Concentration: 12.5 μM, 25 μM, 50 μM
Incubation Time:
Result: Very effectively inhibited the menin-MLL-AF9 interaction in human cells.

Cell Viability Assay[1]

Cell Line: KOPN-8 and MV4;11 cells
Concentration: 0 μM, 0.4 μM, 0.8 μM, 1.2 μM, 1.6 μM
Incubation Time: 72 hours
Result: Showed an effective and dose-dependent growth inhibition in KOPN-8 and MV4;11 cells.

Apoptosis Analysis[1]

Cell Line: MV4;11 cells
Concentration: 12.5 μM, 25 μM, 50 μM
Incubation Time: 48 hours
Result: Resulted in an increase in the number of cells undergoing apoptosis.

RT-PCR[1]

Cell Line: THP-1 cells
Concentration: 6.25 μM, 12.5 μM, 25 μM
Incubation Time: 6 days
Result: Resulted in substantially reduced expression of HOXA9 and MEIS1.
Molecular Weight

375.55

Formula

C₁₈H₂₅N₅S₂

CAS No.

1271738-59-0

SMILES

CC(C)C1=CC2=C(N3CCN(C4=NCC(C)(C)S4)CC3)N=CN=C2S1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (22.18 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6628 mL 13.3138 mL 26.6276 mL
5 mM 0.5326 mL 2.6628 mL 5.3255 mL
10 mM 0.2663 mL 1.3314 mL 2.6628 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.83 mg/mL (2.21 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.83 mg/mL (2.21 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.83 mg/mL (2.21 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

MI-3Menin-MLL inhibitor 3MI3MI 3Epigenetic Reader DomainApoptosisInhibitorinhibitorinhibit

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