1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
  2. Microtubule/Tubulin
    Drug-Linker Conjugates for ADC

Mc-MMAE (Synonyms: Maleimidocaproyl-monomethylauristatin E)

Cat. No.: HY-15741 Purity: 97.23%
Handling Instructions

Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor, is a toxin payload in antibody drug conjugate (ADC).

For research use only. We do not sell to patients.
Mc-MMAE Chemical Structure

Mc-MMAE Chemical Structure

CAS No. : 863971-24-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 1804 In-stock
1 mg USD 660 In-stock
5 mg USD 1176 In-stock
10 mg USD 1800 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor, is a toxin payload in antibody drug conjugate (ADC).

IC50 & Target

Tubulin, Antibody-drug conjugates (ADC)[1]

In Vitro

Synthesis of maleimidocaproyl-MMAE (mc-MMAE) requires the addition of maleimidocaproic acid to a solution of MMAE in dichloromethane followed by the addition of diethyl cyanophosphonate and diisopropylethylamine[1].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.0975 mL 5.4874 mL 10.9748 mL
5 mM 0.2195 mL 1.0975 mL 2.1950 mL
10 mM 0.1097 mL 0.5487 mL 1.0975 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay

Horseradish peroxidase (HRP) is thiolated with 2-iminothiolane and conjugated to mc-MMAE to generate the HRP-MMAE reporter enzyme-drug conjugate. Briefly, a thiolation reaction mixture containing 0.2 mM HRP (8 mg/mL) and 50 mM 2-iminothiolane in 25 mM sodium borate decahydrate (Na2B4O7•10H2O) buffer (pH 9.0) is incubated for 1 hour at 37°C. Unreacted 2-iminothiolane is removed by passage through a PD-10 desalting column equilibrated in PBS (pH 7.4). Peak fractions are pooled and mc-MMAE is coupled to thiolated HRP (HRP-SH) at a molar ratio of 3:1. The final conjugation reaction mixture contained 80 μM HRP-SH (3.2 mg/mL) in sodium borate buffer [50 mM H3BO3, 50 mM NaCl (pH 8.0); 80% v/v] and 240 μM mc-MMAE in ice-cold CH3CN (20% v/v). After 30 minutes on ice, the reaction is terminated with a 20-fold molar excess of free cysteine (4.8 mM) before PD-10 chromatography. Peak fractions containing HRP-MMAE (exchanged into PBS) are pooled and evaluated for extent of modification using the thiol-reactive dye, Alexa Fluor 594 C5 maleimide[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







O=C1N(CCCCCC(N(C)[[email protected]](C(N[[email protected]@H](C(C)C)C(N([[email protected]@H]([[email protected]@H](C)CC)[[email protected]@H](CC(N2[[email protected]]([[email protected]](OC)[[email protected]](C(N[[email protected]@H]([[email protected]](C3=CC=CC=C3)O)C)=O)C)CCC2)=O)OC)C)=O)=O)C(C)C)=O)C(C=C1)=O


4°C, stored under nitrogen


Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 51 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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