1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
  2. Microtubule/Tubulin
    Drug-Linker Conjugates for ADC

Mc-MMAE (Synonyms: Maleimidocaproyl-monomethylauristatin E; Mc MMAE; McMMAE)

Cat. No.: HY-15741 Purity: 97.23%
Data Sheet SDS Handling Instructions

Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor, is a toxin payload in antibody drug conjugate (ADC).

For research use only. We do not sell to patients.
Mc-MMAE Chemical Structure

Mc-MMAE Chemical Structure

CAS No. : 863971-24-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO $1503 In-stock
1 mg $550 In-stock
5 mg $980 In-stock
10 mg $1500 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor, is a toxin payload in antibody drug conjugate (ADC).

IC50 & Target

Tubulin, Antibody-drug conjugates (ADC)[1]

In Vitro

Synthesis of maleimidocaproyl-MMAE (mc-MMAE) requires the addition of maleimidocaproic acid to a solution of MMAE in dichloromethane followed by the addition of diethyl cyanophosphonate and diisopropylethylamine[1].

Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 1.0975 mL 5.4874 mL 10.9748 mL
5 mM 0.2195 mL 1.0975 mL 2.1950 mL
10 mM 0.1097 mL 0.5487 mL 1.0975 mL
Kinase Assay

Horseradish peroxidase (HRP) is thiolated with 2-iminothiolane and conjugated to mc-MMAE to generate the HRP-MMAE reporter enzyme-drug conjugate. Briefly, a thiolation reaction mixture containing 0.2 mM HRP (8 mg/mL) and 50 mM 2-iminothiolane in 25 mM sodium borate decahydrate (Na2B4O7•10H2O) buffer (pH 9.0) is incubated for 1 hour at 37°C. Unreacted 2-iminothiolane is removed by passage through a PD-10 desalting column equilibrated in PBS (pH 7.4). Peak fractions are pooled and mc-MMAE is coupled to thiolated HRP (HRP-SH) at a molar ratio of 3:1. The final conjugation reaction mixture contained 80 μM HRP-SH (3.2 mg/mL) in sodium borate buffer [50 mM H3BO3, 50 mM NaCl (pH 8.0); 80% v/v] and 240 μM mc-MMAE in ice-cold CH3CN (20% v/v). After 30 minutes on ice, the reaction is terminated with a 20-fold molar excess of free cysteine (4.8 mM) before PD-10 chromatography. Peak fractions containing HRP-MMAE (exchanged into PBS) are pooled and evaluated for extent of modification using the thiol-reactive dye, Alexa Fluor 594 C5 maleimide[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.








Powder4°C, stored under nitrogen


Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 51 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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