N-Acetylcysteine amide
Based on 14 publication(s) in Google Scholar
N-Acetylcysteine amide is a cell membranes and blood brain barrier permeant thiol antioxidant and neuroprotective agent, reduces ROS production.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 38520-57-9
- Formula: C5H10N2O2S
- Molecular Weight:162.21
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) N-Acetylcysteine amide
More- Nat Metab. 2021 Sep;3(9):1242-1258. [Abstract]
- Autophagy. 2024 Nov;20(11):2460-2477. [Abstract]
- Int J Mol Med. 2019 Dec;44(6):2189-2200. [Abstract]
- Int J Mol Med. 2019 Aug;44(2):447-456. [Abstract]
- Food Funct. 2025 Aug 26;16(17):6786-6799. [Abstract]
- Chem Biol Interact. 2024 Jul 3:111133. [Abstract]
- Toxicology. 2019 Apr 15:418:22-31. [Abstract]
- Sci Rep. 2026 Mar 20;16(1):14300. [Abstract]
- J Cell Mol Med. 2020 Jan;24(2):1332-1344. [Abstract]
- iScience. 2026 Jun 3;29(6):115796. [Abstract]
- J Funct Foods. 57 (2019) 255-265.
- Nanotoxicology. 2020 Jun;14(5):667-682. [Abstract]
- J Neurosci Res. 2019 Dec;97(12):1689-1705. [Abstract]
- Toxicol In Vitro. 2017 Oct:44:57-65. [Abstract]
Customer Validation & Images
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WB
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Cell Imaging/Staining
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Flow Cytometry
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IP
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Flow Cytometry
Biological Activity
N-Acetylcysteine amide shows no obvious effect on the viability of H9c2 cells treated with doxorubicin (DOX) at < 1 mM, but causes significant cytotoxicity at 10-20 mM. N-Acetylcysteine amide (750 μM) reduces the ROS levle and lipid peroxidation induced by DOX, and restores GSH/GSSG ratio and activities of antioxidant enzymes, such as catalase (CAT), gluthathione peroxidase (GPx), gluthathione reductase (GR)[1]. N-Acetylcysteine amide (1 mM) protects the human brain microvascular endothelial (HBMVEC) from methamphetamine (METH)- induced cell death[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 38520-57-9
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Appearance Solid
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Molecular Weight 162.21
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Formula C5H10N2O2S
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Color White to off-white
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SMILES
CC(N[C@@H](CS)C(N)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (14)
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Journal Impact Factor
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Most Recent
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Nat Metab
A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidation. [Abstract]2021 Sep;3(9):1242-1258. PMID: 34504353
N-Acetylcysteine amide purchased from MedChemExpress. Usage Cited in: Nat Metab. 2021 Sep;3(9):1242-1258. [Abstract]
Old (day 50) wild-type flies, fed with or without N-Acetylcysteine amide (AD4, 40 μg/ml) from day 30–50, were IPed. n=5 for DMiro Input and 4 for the rest. p=0.0286 and 0.0159.
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Autophagy
(-)-Epigallocatechin 3-gallate protects pancreatic β-cell against excessive autophagy-induced injury through promoting FTO degradation. [Abstract]2024 Nov;20(11):2460-2477. PMID: 38910554
N-Acetylcysteine amide purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Nov;20(11):2460-2477. [Abstract]
Western blot analysis of LC3 and SQSTM1 in control and FTO overexpressed MIN6 cells treated with or without 1 mmol/l N-Acetylcysteine amide (NAC) for 48 h.
N-Acetylcysteine amide purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Nov;20(11):2460-2477. [Abstract]
Representative TEM images of pancreatic β cells obtained from ctrl mice and βNR3C1 mice with or without 1 mmol/l N-Acetylcysteine amide (NAC) for 48 h. The number of autophagic structures was quantified on the right (n = 20). Scale bar: 1 μm.
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Int J Mol Med
Glutamine affects T24 bladder cancer cell proliferation by activating STAT3 through ROS and glutaminolysis. [Abstract]2019 Dec;44(6):2189-2200. PMID: 31661119 -
Int J Mol Med
Effects of transcription factor EB on oxidative stress and apoptosis induced by high glucose in podocytes. [Abstract]2019 Aug;44(2):447-456. PMID: 31173156 -
Food Funct
Combination of resveratrol and epirubicin to overcome chemoresistance in lung adenocarcinoma organoids: synergistic effects and translational implications. [Abstract]2025 Aug 26;16(17):6786-6799. PMID: 40755405 -
Chem Biol Interact
Bavachinin, a main compound of Psoraleae Fructus, facilitates GSDMD-mediated pyroptosis and causes hepatotoxicity in mice. [Abstract]2024 Jul 3:111133. PMID: 38969277
N-Acetylcysteine amide purchased from MedChemExpress. Usage Cited in: Chem Biol Interact. 2024 Jul 3:111133. [Abstract]
Percentage mtROS production by LPS-primed BMDMs stimulated with bavachinin, N-Acetylcysteine amide (NAC) (2.5 mmol/L), or bavachinin plus NAC (2.5 mmol/L), followed by MitoSOX red stained and flow cytometry to detect mtROS production.
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Toxicology
Ginsenoside Rk1 induces cell cycle arrest and apoptosis in MDA-MB-231 triple negative breast cancer cells. [Abstract]2019 Apr 15:418:22-31. PMID: 30797898 -
Sci Rep
2026 Mar 20;16(1):14300. PMID: 41862552 -
J Cell Mol Med
Fuziline alleviates isoproterenol-induced myocardial injury by inhibiting ROS-triggered endoplasmic reticulum stress via PERK/eIF2α/ATF4/Chop pathway. [Abstract]2020 Jan;24(2):1332-1344. PMID: 31811750
N-Acetylcysteine amide purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jan;24(2):1332-1344. [Abstract]
Inhibition of endoplasmic reticulum stress by fuziline is regulated by the production of ROS. The effect of N-Acetylcysteine amide (NAC) (3 mmol/L), GSK2606414 and fuziline on the production of ROS was measured by DCFH‐DA staining via flow cytometry.
N-Acetylcysteine amide purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jan;24(2):1332-1344. [Abstract]
The effect of N-Acetylcysteine amide (NAC) (3 mmol/L), GSK2606414 and fuziline on cell apoptosis was measured by Annexin‐FITC/PI staining via flow cytometry.
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iScience
Dim light at night induces cardiac injury in zebrafish embryos via disrupted chloride homeostasis. [Abstract]2026 Jun 3;29(6):115796. PMID: 42291255 -
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Nanotoxicology
Graphene oxide nanoparticles induce hepatic dysfunction through the regulation of innate immune signaling in zebrafish ( Danio rerio). [Abstract]2020 Jun;14(5):667-682. PMID: 32141807 -
J Neurosci Res
Enhanced mitochondrial inhibition by 3,4-dihydroxyphenyl-acetaldehyde (DOPAL)-oligomerized α-synuclein. [Abstract]2019 Dec;97(12):1689-1705. PMID: 31420910 -
Toxicol In Vitro
Propofol inhibits invasion and proliferation of C6 glioma cells by regulating the Ca2+ permeable AMPA receptor-system xc- pathway. [Abstract]2017 Oct:44:57-65. PMID: 28663055
N-Acetylcysteine amide purchased from MedChemExpress. Usage Cited in: Toxicol In Vitro. 2017 Oct:44:57-65. [Abstract]
A Transwell invasion assay is used to measure the effect of propofol and N-acetylcysteine (NAC).
Solvent & Solubility
DMSO : ≥ 100 mg/mL (616.48 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (616.48 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
To choose a sublethal concentration of N-Acetylcysteine amide and N-acetylcysteine for the study on their ability to protect cells from doxorubicin (DOX)-induced toxicity, H9c2 cells are exposed with N-Acetylcysteine amide or N-acetylcysteine at 0.25 mM, 0.50 mM, 0.75 mM, 1 mM, 2 mM, 5 mM, 10 mM, and 20 mM for 24 h. Untreated cells are used as the control for each experiment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats[2]
In order to assess mitochondrial respiration and glutathione content following traumatic brain injury (TBI), rats are randomly divided into three groups (n = 5 animals/group). (I.) N-Acetylcysteine amide group receives multiple bolus IP injections of N-Acetylcysteine amide (150 mg/kg) immediately after 5 minutes and then every 6 hours up to 24 hrs post-injury. (II.) Vehicle group receives equivalent v/v saline at 5 minutes and every 6 hours (6, 12, 18, 24 hrs) up to 24 hrs post-injury. (III.) Sham injured group animals do not receive any drug treatment. At 25 hrs post-injury, all animals are euthanized and mitochondria are isolated from the ipsilateral cortical hemisphere (6 mm punch) to carry out measurements of mitochondrial respiration and glutathione content[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Shi R, et al. N-acetylcysteine amide decreases oxidative stress but not cell death induced by doxorubicin in H9c2 cardiomyocytes. BMC Pharmacol. 2009 Apr 15;9:7. [Content Brief]
[2]. Pandya JD, et al. N-acetylcysteine amide confers neuroprotection, improves bioenergetics and behavioral outcome following TBI. Exp Neurol. 2014 Jul;257:106-13. [Content Brief]
[3]. Zhang X, et al. N-Acetylcysteine amide protects against methamphetamine-induced oxidative stress and neurotoxicity in immortalized human brain endothelial cells. Brain Res. 2009 Jun 12;1275:87-95. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 6.1648 mL | 30.8242 mL | 61.6485 mL | 154.1212 mL |
| 5 mM | 1.2330 mL | 6.1648 mL | 12.3297 mL | 30.8242 mL | |
| 10 mM | 0.6165 mL | 3.0824 mL | 6.1648 mL | 15.4121 mL | |
| 15 mM | 0.4110 mL | 2.0549 mL | 4.1099 mL | 10.2747 mL | |
| 20 mM | 0.3082 mL | 1.5412 mL | 3.0824 mL | 7.7061 mL | |
| 25 mM | 0.2466 mL | 1.2330 mL | 2.4659 mL | 6.1648 mL | |
| 30 mM | 0.2055 mL | 1.0275 mL | 2.0549 mL | 5.1374 mL | |
| 40 mM | 0.1541 mL | 0.7706 mL | 1.5412 mL | 3.8530 mL | |
| 50 mM | 0.1233 mL | 0.6165 mL | 1.2330 mL | 3.0824 mL | |
| 60 mM | 0.1027 mL | 0.5137 mL | 1.0275 mL | 2.5687 mL | |
| 80 mM | 0.0771 mL | 0.3853 mL | 0.7706 mL | 1.9265 mL | |
| 100 mM | 0.0616 mL | 0.3082 mL | 0.6165 mL | 1.5412 mL |
N-Acetylcysteine amide Related Classifications
HY-110256 Related Classifications
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