1. Biochemical Assay Reagents
  2. O-Propargyl-Puromycin

O-Propargyl-Puromycin (Synonyms: O-Propargylpuromycin)

Cat. No.: HY-15680 Purity: 95.75% ee.: 99.11%
Handling Instructions

O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor.

For research use only. We do not sell to patients.

O-Propargyl-Puromycin Chemical Structure

O-Propargyl-Puromycin Chemical Structure

CAS No. : 1416561-90-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 1295 In-stock
Estimated Time of Arrival: December 31
500 μg USD 192 In-stock
Estimated Time of Arrival: December 31
1 mg USD 312 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1188 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE O-Propargyl-Puromycin

    O-Propargyl-Puromycin purchased from MCE. Usage Cited in: Elife. 2015 Oct 24;4. pii: e09436.

    Polypeptide synthetic activity is determined by OP-puromycin staining.
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    Description

    O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor.

    In Vitro

    O-propargyl-puromycin can be used to image and affinity-purify nascent proteins in cells and in animals. O-propargyl-puromycin inhibits protein synthesis, both in reticulocyte lysates and in cultured cells, displaying a potency two- to threefold lower than that of unmodified puromycin. O-propargyl-puromycin forms covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. [1].

    In Vivo

    Tissues from uninjected mice shows low nonspecific staining, whereas tissues from O-propargyl-puromycin-injected mice display specific patterns of O-propargyl-puromycin incorporation into nascent proteins. In the small intestine, translation is strongest in cells in the crypts and at the base of intestinal villi, consistent with the high proliferative and secretory activity of these cells. The stain is particularly strong in Paneth cells, which are located close to the base of the crypts and are filled with secretory vesicles. The intense O-propargyl-puromycin labeling of vesicles in Paneth cells suggests that prematurely terminated, O-propargyl-puromycin-conjugated secretory proteins are translocated into the endoplasmic reticulum (ER) lumen[1].

    Molecular Weight

    495.53

    Formula

    C₂₄H₂₉N₇O₅

    CAS No.

    1416561-90-4

    SMILES

    CN(C)C1=C2C(N([[email protected]]3[[email protected]](O)[[email protected]](NC([[email protected]@H](N)CC4=CC=C(OCC#C)C=C4)=O)[[email protected]@H](CO)O3)C=N2)=NC=N1

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 31 mg/mL (62.56 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0180 mL 10.0902 mL 20.1804 mL
    5 mM 0.4036 mL 2.0180 mL 4.0361 mL
    10 mM 0.2018 mL 1.0090 mL 2.0180 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Animal Administration
    [1]

    Mice: 100 μL of a 20 mM solution of O-propargyl-puromycin in PBS are injected intraperitoneally into a 3-wk-old mouse, and a mouse injected with 100 μL of PBS is used as negative control. Various organs are harvested after 1 h and are fixed in formalin overnight. Organ fragments are embedded in paraffin, sectioned, and ished with xylene to remove the paraffin. After ishing with ethanol and rehydration in TBS, the tissue sections are stained with 20 μM tetramethylrhodamine(TMR)-azide. The tissue sections are counterstained with Hoechst, mounted in standard mounting media, and are then imaged by fluorescence microscopy and DIC[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Product Name:
    O-Propargyl-Puromycin
    Cat. No.:
    HY-15680
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