O-Propargyl-Puromycin
Based on 33 publication(s) in Google Scholar
O-Propargyl-Puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor. O-Propargyl-Puromycin is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity: 99.71%
- CAS No.: 1416561-90-4
- Formula: C24H29N7O5
- Molecular Weight:495.53
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) O-Propargyl-Puromycin
More- Nature. 2022 Mar;603(7901):477-481. [Abstract]
- Cell. 2026 Jun 10:S0092-8674(26)00581-7. [Abstract]
- Cell. 2026 Apr 30;189(9):2684-2699.e21. [Abstract]
- Cell Metab. 2025 Sep 16:S1550-4131(25)00381-X. [Abstract]
- Cell Metab. 2023 Jun 6;35(6):1009-1021.e9. [Abstract]
- Cell Stem Cell. 2021 Jul 1;28(7):1275-1290.e9. [Abstract]
- Cancer Res. 2026 Feb 24. [Abstract]
- Nat Commun. 2025 May 27;16(1):4920. [Abstract]
- Sci Adv. 2026 May 22;12(21):eadz7896. [Abstract]
- Sci Adv. 2025 Sep 12;11(37):eadx4511. [Abstract]
- Sci Adv. 2024 Jan 12;10(2):eadh9871. [Abstract]
- J Exp Med. 2022 Dec 5;219(12):e20220685. [Abstract]
- J Exp Med. 2021 Oct 4;218(10):e20210227. [Abstract]
- Cancer Biol Med. 2021 Mar 12;19(1):104-119. [Abstract]
- Cell Rep. 2025 Nov 25;44(11):116496. [Abstract]
- Cell Rep. 2024 Oct 15;43(11):114864. [Abstract]
- Elife. 2015 Oct 24;4:e09436. [Abstract]
- Mol Cell Proteomics. 2025 Feb 4:100914. [Abstract]
- Int J Mol Sci. 2025 May 16;26(10):4801. [Abstract]
- Int Immunopharmacol. 2024 Jun 24:138:112518. [Abstract]
- STAR Protoc. 2023 Mar 31;4(2):102195. [Abstract]
- STAR Protoc. 2020 Sep 18;1(2):100103. [Abstract]
- bioRxiv. 2026 Apr 18.
- bioRxiv. 2026 Mar 9.
- bioRxiv. 2025 Sep 25:2025.09.24.678136. [Abstract]
- bioRxiv. 2025 March 17.
- Patent. US20240392239A1.
- Patent. US20240301413A1.
- Research Square Preprint. 2023 Oct 16.
- bioRxiv. 2023 Mar 5.
- bioRxiv. 2023 Jan 23.
- bioRxiv. January 14, 2022.
- Methods Mol Biol. 2021:2318:255-266. [Abstract]
Customer Validation & Images
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Flow Cytometry
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Flow Cytometry
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Flow Cytometry
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Flow Cytometry
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IF
Biological Activity
O-propargyl-puromycin can be used to image and affinity-purify nascent proteins in cells and in animals. O-propargyl-puromycin inhibits protein synthesis, both in reticulocyte lysates and in cultured cells, displaying a potency two- to threefold lower than that of unmodified puromycin. O-propargyl-puromycin forms covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1416561-90-4
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Appearance Solid
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Molecular Weight 495.53
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Formula C24H29N7O5
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Color White to off-white
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SMILES
CN(C)C1=C2C(N([C@H]3[C@H](O)[C@H](NC([C@@H](N)CC4=CC=C(OCC#C)C=C4)=O)[C@@H](CO)O3)C=N2)=NC=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (33)
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Journal Impact Factor
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Most Recent
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Nature
2022 Mar;603(7901):477-481. PMID: 35264789 -
Cell
2026 Jun 10:S0092-8674(26)00581-7. PMID: 42269604 -
Cell
2026 Apr 30;189(9):2684-2699.e21. PMID: 41763199 -
Cell Metab
OGFOD1 enables AML chemo- and nutrient stress resistance by regulating protein synthesis. [Abstract]2025 Sep 16:S1550-4131(25)00381-X. PMID: 40961937
O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Sep 16:S1550-4131(25)00381-X. [Abstract]
Mice were injected with MLL-AF9 AML, switched to a BCAA-free diet and treated with 5 + 3. At the point of maximum response, OPP was administered by i.p. injection (50 mg/kg) and the mouse was sacrificed after 1 h. BM cells were isolated, fixed, and permeabilized before an azide-alkyne iClick-iT reaction was performed to fluorescently label nascent peptides with OPP. OPP incorporation was measured by flow cytometry.
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Cell Metab
Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness. [Abstract]2023 Jun 6;35(6):1009-1021.e9. PMID: 37084733 -
Cell Stem Cell
HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. [Abstract]2021 Jul 1;28(7):1275-1290.e9. PMID: 33711283
O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2021 Jul 1;28(7):1275-1290.e9. [Abstract]
Global protein synthesis rates of various TF-1 cells stably expressing control shLuc, single or double knockdown of HECTD1 and ZNF622 were measured using OP-Puro assay. OP-Puro was added to cell culture at the final concentration of 20uM for 1 hour at a 37°C incubator.
O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2021 Jul 1;28(7):1275-1290.e9. [Abstract]
Representative histogram plot of protein synthesis rate in BM HSCs of 5-FU challenged mice as determined by in vivo OP-Puro assay. To detect protein synthesis rate in vivo, primary or stressed mice were injected intraperitoneally with OP-Puro (50mg/kg, pH6.4–6.6 in PBS) for 1 hour before euthanasia.
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Cancer Res
Ribonuclease 5/Angiogenin Suppresses Intestinal Tumor Initiation by Maintaining Crypt Homeostasis. [Abstract]2026 Feb 24. PMID: 41734376 -
Nat Commun
WNK1 signalling regulates amino acid transport and mTORC1 activity to sustain acute myeloid leukaemia growth. [Abstract]2025 May 27;16(1):4920. PMID: 40425534
O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 27;16(1):4920. [Abstract]
Protein synthesis rates as measured by incorporation of OP-puro in Wnk1fl/− MA9 leukaemia cells treated with 10 µM Compound 12 at the indicated time points. Cells were labelled with 10 µM OP-Puro for 30 min.
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Sci Adv
Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress. [Abstract]2026 May 22;12(21):eadz7896. PMID: 42172329 -
Sci Adv
The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles. [Abstract]2025 Sep 12;11(37):eadx4511. PMID: 40938992 -
Sci Adv
N-acetyltransferase NAT10 controls cell fates via connecting mRNA cytidine acetylation to chromatin signaling. [Abstract]2024 Jan 12;10(2):eadh9871. PMID: 38215194 -
J Exp Med
Mitochondria transfer mediates stress erythropoiesis by altering the bioenergetic profiles of early erythroblasts through CD47. [Abstract]2022 Dec 5;219(12):e20220685. PMID: 36112140 -
J Exp Med
2021 Oct 4;218(10):e20210227. PMID: 34424266 -
Cancer Biol Med
SNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA. [Abstract]2021 Mar 12;19(1):104-119. PMID: 33710804 -
Cell Rep
A generalized strategy to kill leukemic cells by targeting the regulatory systems governing mitochondrial membrane potential. [Abstract]2025 Nov 25;44(11):116496. PMID: 41166305 -
Cell Rep
Defective ribosome assembly impairs leukemia progression in a murine model of acute myeloid leukemia. [Abstract]2024 Oct 15;43(11):114864. PMID: 39412990 -
Elife
Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma. [Abstract]2015 Oct 24;4:e09436. PMID: 26499495
O-Propargyl-Puromycin purchased from MedChemExpress. Usage Cited in: Elife. 2015 Oct 24;4:e09436. [Abstract]
Polypeptide synthetic activity is determined by OP-puromycin staining.
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Mol Cell Proteomics
Proteogenomic Profiling Reveals Small ORFs and Functional Microproteins in Activated T Cells. [Abstract]2025 Feb 4:100914. PMID: 39914663 -
Int J Mol Sci
A Coordinated Translational Control Mediated by eEF2 Phosphorylation Safeguards Erythroid Differentiation. [Abstract]2025 May 16;26(10):4801. PMID: 40429942 -
Int Immunopharmacol
Targeting lipid peroxidation-associated ferroptosis suppresses lung carcinoma progression by regulating cell cycle arrest. [Abstract]2024 Jun 24:138:112518. PMID: 38917528 -
STAR Protoc
Metabolic labeling and LC-MS/MS-based identification of interleukin-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum. [Abstract]2023 Mar 31;4(2):102195. PMID: 37004159 -
STAR Protoc
Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro. [Abstract]2020 Sep 18;1(2):100103. PMID: 33000005 -
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bioRxiv
Differentiation stage-specific use of cap-independent and cap-dependent translation initiation in hematopoiesis. [Abstract]2025 Sep 25:2025.09.24.678136. PMID: 41040404 -
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Methods Mol Biol
2021:2318:255-266. PMID: 34019295
Solvent & Solubility
DMSO : 100 mg/mL (201.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Mice: 100 μL of a 20 mM solution of O-propargyl-puromycin in PBS are injected intraperitoneally into a 3-wk-old mouse, and a mouse injected with 100 μL of PBS is used as negative control. Various organs are harvested after 1 h and are fixed in formalin overnight. Organ fragments are embedded in paraffin, sectioned, and ished with xylene to remove the paraffin. After ishing with ethanol and rehydration in TBS, the tissue sections are stained with 20 μM tetramethylrhodamine(TMR)-azide. The tissue sections are counterstained with Hoechst, mounted in standard mounting media, and are then imaged by fluorescence microscopy and DIC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0180 mL | 10.0902 mL | 20.1804 mL | 50.4510 mL |
| 5 mM | 0.4036 mL | 2.0180 mL | 4.0361 mL | 10.0902 mL | |
| 10 mM | 0.2018 mL | 1.0090 mL | 2.0180 mL | 5.0451 mL | |
| 15 mM | 0.1345 mL | 0.6727 mL | 1.3454 mL | 3.3634 mL | |
| 20 mM | 0.1009 mL | 0.5045 mL | 1.0090 mL | 2.5226 mL | |
| 25 mM | 0.0807 mL | 0.4036 mL | 0.8072 mL | 2.0180 mL | |
| 30 mM | 0.0673 mL | 0.3363 mL | 0.6727 mL | 1.6817 mL | |
| 40 mM | 0.0505 mL | 0.2523 mL | 0.5045 mL | 1.2613 mL | |
| 50 mM | 0.0404 mL | 0.2018 mL | 0.4036 mL | 1.0090 mL | |
| 60 mM | 0.0336 mL | 0.1682 mL | 0.3363 mL | 0.8409 mL | |
| 80 mM | 0.0252 mL | 0.1261 mL | 0.2523 mL | 0.6306 mL | |
| 100 mM | 0.0202 mL | 0.1009 mL | 0.2018 mL | 0.5045 mL |
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