2. Cytoskeleton Epigenetics TGF-beta/Smad
  3. MARCKS PKC
  4. TPPB

TPPB is a cell-permeable benzolactam-derived protein kinase C (PKC) activator with a Ki of 11.9 nM.

For research use only. We do not sell to patients.

CAS No. : 497259-23-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

TPPB Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE TPPB

ELISA
WB
RT-PCR
Flow Cytometry

    TPPB purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 12;16(1):11092.  [Abstract]

    Western blotting analysis of RKIP expression in PA-stimulated HepG2 cells with MG-132 (10 μM; 12 h) treatment.

    TPPB purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 12;16(1):11092.  [Abstract]

    MG-132 (10 μM; 6 h) rescued the decreased expression of RKIP in HepG2 cells with ER stress inducer (tunicamycin, TM; thapsigargin, Tha).

    TPPB purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 12;16(1):11092.  [Abstract]

    MG-132 (10 μM; 12 h) rescued the decreased expression of RKIPC133A protein in HEK293T cells.

    TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609.  [Abstract]

    Effect of TPPB (0.5 μM; 48 h) on the reactivation of latent HIV in U1 and ACH-2 cells.

    TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609.  [Abstract]

    TPPB (0.5 μM; 48 h) reactivated latent HIV in OM-10.1, ACH-2, and U1 cells, as measured using HIV RNA quantification.

    TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609.  [Abstract]

    Effect of TPPB (0.5 μM; 48 h) on the reactivation of latent HIV in OM-10.1 cells.

    TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609.  [Abstract]

    TPPB (0.5 μM; 24-48 h) induced gp120 expression in latently infected cells (OM-10.1 cells and ACH-2 cells).

    TPPB purchased from MedChemExpress. Usage Cited in: Viruses. 2020 Jun 3;12(6):609.  [Abstract]

    Representative overlay and single peaks of CD4 expression of CD4+ T-cells treated with TPPB (0.5 μM; 24 h).

    View All PKC Isoform Specific Products:

    View All Isoforms
    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TPPB is a cell-permeable benzolactam-derived protein kinase C (PKC) activator with a Ki of 11.9 nM.

    IC50 & Target[1]

    PKC

    11.9 nM (Ki)

    In Vitro

    By use of a cell line derived from an Alzheimer’s disease patient, significant enhancement of sAPPα secretion is achieved at 1 μM concentration for TPPB (Compound 5e)[1]. TPPB has a role against Aβ25-35-induced neurotoxicity in PC12 cells. TPPB at concentration of 1 μM could antagonize Aβ25-35 induced cell damage. TPPB could increase the phosphorylation of Akt, PKC, MARCKS and MAPK, which are inhibited by Aβ25-35 treatment. TPPB inhibits the activation of caspase-3 induced by Aβ25-35[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TPPB Related Antibodies
    In Vivo

    TPPB is evaluated for induction of hyperplasia after topical application to the shaved backs of outbred Sencar mice and shows a modest response at 300 μg[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    501.54

    Formula

    C27H30F3N3O3
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(NC1=CC=C(N(C)[C@@H](C(C)C)C(N[C@H](CO)C2)=O)C2=C1)/C=C/C=C/C3=CC=C(C(F)(F)F)C=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (249.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9939 mL 9.9693 mL 19.9386 mL
    5 mM 0.3988 mL 1.9939 mL 3.9877 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.25 mg/mL (4.49 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.25 mg/mL (4.49 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.92%

    SDS (251 KB)

    COA (246 KB) LCMS (91 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    24 h after plating 2×104 pheochromocytoma PC12 cells in each well of a 96 well plate, cells are incubated with TPPB at a series of concentration (0.1, 0.5, 1, 5, 10, 20 μM). Twelve to 24 h later, the original media is replaced with media containing MTT at a final concentration of 0.5 g/L for 4 h. Cell viability is evaluated with MTT assays[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice: Mice are 7 weeks old at the beginning of the treatments and are in the resting phase of the hair cycle. TPPB is applied once or else are applied twice weekly for a total of four applications. Two animals are treated at each dose of compound, and 72 h after the last application, the animals are euthanized. Two portions of treated skin are removed from each animal, fixed in neutral buffered formalin, and stained with hematoxylin and eosin for histological analysis[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9939 mL 9.9693 mL 19.9386 mL 49.8465 mL
    5 mM 0.3988 mL 1.9939 mL 3.9877 mL 9.9693 mL
    10 mM 0.1994 mL 0.9969 mL 1.9939 mL 4.9846 mL
    15 mM 0.1329 mL 0.6646 mL 1.3292 mL 3.3231 mL
    20 mM 0.0997 mL 0.4985 mL 0.9969 mL 2.4923 mL
    25 mM 0.0798 mL 0.3988 mL 0.7975 mL 1.9939 mL
    30 mM 0.0665 mL 0.3323 mL 0.6646 mL 1.6615 mL
    40 mM 0.0498 mL 0.2492 mL 0.4985 mL 1.2462 mL
    50 mM 0.0399 mL 0.1994 mL 0.3988 mL 0.9969 mL
    60 mM 0.0332 mL 0.1662 mL 0.3323 mL 0.8308 mL
    80 mM 0.0249 mL 0.1246 mL 0.2492 mL 0.6231 mL
    100 mM 0.0199 mL 0.0997 mL 0.1994 mL 0.4985 mL
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    TPPB Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TPPB497259-23-1MARCKSPKCMyristoylated alanine-rich C kinase substrateProtein kinase CInhibitorinhibitorinhibit

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