1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
  2. Microtubule/Tubulin
    ADC Cytotoxin

D8-MMAE (Synonyms: D8-Monomethyl auristatin E; D8-Vedotin)

Cat. No.: HY-15162A Purity: 98.31%
Handling Instructions

D8-MMAE is a deuterated form of MMAE, which is a microtubule-disrupting agent.

For research use only. We do not sell to patients.
D8-MMAE Chemical Structure

D8-MMAE Chemical Structure

CAS No. : 2070009-72-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 4792 In-stock
1 mg USD 1320 In-stock
5 mg USD 3000 In-stock
10 mg USD 5640 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Other Forms of D8-MMAE:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


D8-MMAE is a deuterated form of MMAE, which is a microtubule-disrupting agent.

IC50 & Target


In Vitro

Antibody-drug conjugates (ADC) comprise targeting antibodies armed with potent small-molecule payloads. ADCs are generated to target different receptors on the anaplastic large cell lymphoma line L-82, but delivered the same cytotoxic payload (monomethyl auristatin E, MMAE), and the intracellular concentration of released MMAE correlated with in vitro ADC-mediated cytotoxicity independent of target expression or drug:antibody ratios. LC-MS is used to measure the concentration of MMAE in a parallel cohort of L-82 tumors with an identical treatment regimen. Although tumor volume is not different among treatment groups 3 days after dose, the intratumoral MMAE measurement reveals two patterns. First, intratumoral MMAE concentration increases proportionally to the ADC dose, which correspondes to stronger antitumor activity. Second, the intratumoral MMAE concentration obtained from treatment with both cOKT9-vcMMAE and cAC10-vcMMAE is similar at each dose, consistent with the observation that tumor responded similarly to these two ADCs[1].

In Vivo

Intratumoral MMAE concentrations consistently correlates with the extent of tumor growth inhibition in tumor xenograft models. IHC analysis reveals that nonbinding control-treated tumors consist of both CD30+ and CD30-cells, presumably because they do not kill either CD30+ or CD30- Karpas 299 cells. Only CD30- cells are found in cAC10-vcMMAF-treated tumors, illustrating that cAC10-vcMMAF eliminates most CD30+ cells. Interestingly, the two tumors that relapses from cAC10-vcMMAE treatment are also found to be CD30- by the end of study, indicating a small fraction of CD30- cells might have escaped from bystander killing in these two remaining tumors[1].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.3774 mL 6.8868 mL 13.7735 mL
5 mM 0.2755 mL 1.3774 mL 2.7547 mL
10 mM 0.1377 mL 0.6887 mL 1.3774 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay

Cell pellets are collected 24 hours after ADC treatment. Cell count, diameter, and circularity are determined on Vi-Cell Counter. MMAE extraction and quantification method is performed. Briefly, tumors or cell pellets are homogenized with methanol and acetonitrile containing internal standard (d8-MMAE for MMAE detection and 13C-MMAF for MMAF detection). The homogenates are centrifuged at 10,000 rpm to precipitate protein and protein-bound payloads. The supernatant is then subjected to solid phase extraction, and signals of MMAE and MMAF are detected by LC-MS[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







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4°C, stored under nitrogen


Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 40 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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