1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
  2. Microtubule/Tubulin
    ADC Cytotoxin
  3. D8-MMAE

D8-MMAE (Synonyms: D8-Monomethyl auristatin E)

Cat. No.: HY-15162A Purity: 99.29%
Handling Instructions

D8-MMAE is a deuterated labeled MMAE, a potent mitotic inhibitor and a tubulin inhibitor.

For research use only. We do not sell to patients.

D8-MMAE Chemical Structure

D8-MMAE Chemical Structure

CAS No. : 2070009-72-0

Size Price Stock Quantity
1 mg USD 1320 In-stock
Estimated Time of Arrival: December 31
5 mg USD 3000 In-stock
Estimated Time of Arrival: December 31
10 mg USD 5640 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of D8-MMAE:

Top Publications Citing Use of Products

Publications Citing Use of MCE D8-MMAE

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review


D8-MMAE is a deuterated labeled MMAE, a potent mitotic inhibitor and a tubulin inhibitor.

IC50 & Target



In Vitro

Antibody-drug conjugates (ADC) comprise targeting antibodies armed with potent small-molecule payloads. ADCs are generated to target different receptors on the anaplastic large cell lymphoma line L-82, but delivered the same cytotoxic payload (monomethyl auristatin E, MMAE), and the intracellular concentration of released MMAE correlated with in vitro ADC-mediated cytotoxicity independent of target expression or drug:antibody ratios. LC-MS is used to measure the concentration of MMAE in a parallel cohort of L-82 tumors with an identical treatment regimen. Although tumor volume is not different among treatment groups 3 days after dose, the intratumoral MMAE measurement reveals two patterns. First, intratumoral MMAE concentration increases proportionally to the ADC dose, which correspondes to stronger antitumor activity. Second, the intratumoral MMAE concentration obtained from treatment with both cOKT9-vcMMAE and cAC10-vcMMAE is similar at each dose, consistent with the observation that tumor responded similarly to these two ADCs[1].

In Vivo

Intratumoral MMAE concentrations consistently correlates with the extent of tumor growth inhibition in tumor xenograft models. IHC analysis reveals that nonbinding control-treated tumors consist of both CD30+ and CD30-cells, presumably because they do not kill either CD30+ or CD30- Karpas 299 cells. Only CD30- cells are found in cAC10-vcMMAF-treated tumors, illustrating that cAC10-vcMMAF eliminates most CD30+ cells. Interestingly, the two tumors that relapses from cAC10-vcMMAE treatment are also found to be CD30- by the end of study, indicating a small fraction of CD30- cells might have escaped from bystander killing in these two remaining tumors[1].

Molecular Weight







CC[[email protected]](C)[[email protected]@H]([[email protected]@H](CC(N1[[email protected]@H](CCC1)[[email protected]@H]([[email protected]@H](C)C(N[[email protected]](C)[[email protected]@H](O)C2=CC=CC=C2)=O)OC)=O)OC)N(C([[email protected]@](C(C([2H])([2H])[2H])(C([2H])([2H])[2H])[2H])([2H])NC([[email protected]](C(C)C)NC)=O)=O)C


Room temperature in continental US; may vary elsewhere.


4°C, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (137.74 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3774 mL 6.8868 mL 13.7735 mL
5 mM 0.2755 mL 1.3774 mL 2.7547 mL
10 mM 0.1377 mL 0.6887 mL 1.3774 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.44 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.44 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.44 mM); Clear solution

*All of the co-solvents are provided by MCE.
Kinase Assay

Cell pellets are collected 24 hours after ADC treatment. Cell count, diameter, and circularity are determined on Vi-Cell Counter. MMAE extraction and quantification method is performed. Briefly, tumors or cell pellets are homogenized with methanol and acetonitrile containing internal standard (d8-MMAE for MMAE detection and 13C-MMAF for MMAF detection). The homogenates are centrifuged at 10,000 rpm to precipitate protein and protein-bound payloads. The supernatant is then subjected to solid phase extraction, and signals of MMAE and MMAF are detected by LC-MS[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2


D8-MMAED8-Monomethyl auristatin EMicrotubule/TubulinADC CytotoxinInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product name



Applicant name *


Email address *

Phone number *


Organization name *

Country or Region *


Requested quantity *


Bulk Inquiry

Inquiry Information

Product name:
Cat. No.:
MCE Japan Authorized Agent: