2. Apoptosis
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  4. Erucin

Erucin 

Cat. No.: HY-121323
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Erucin (ERU) is an isothiocyanate particularly abundant in arugula. Erucin shows anticancer, neuroprotective, and anti-inflammatory activities.

For research use only. We do not sell to patients.

Erucin Chemical Structure

Erucin Chemical Structure

CAS No. : 4430-36-8

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Description

Erucin (ERU) is an isothiocyanate particularly abundant in arugula. Erucin shows anticancer, neuroprotective, and anti-inflammatory activities[1][2][3][4].

In Vitro

Erucin (ERU) (0-100 μM) releases H2S and inhibits cell viability in AsPC‐1 cells in a concentration-dependent manner[1].
Erucin inhibits cell migration and altered the AsPC‐1 cell cycle, reducing G0/G1 phase and increasing G2/M and S phases[1].
Erucin (30 μM, 72 h) induces AsPC‐1 cell apoptosis and inhibits cell migration[1].
Erucin reduces levels of phosphorylated ERK1/2 in AsPC‐1 cells[1].
Erucin (0-200 μM, 24 h) shows antiproliferative activity with an IC50 of 97.7 µM in A549 cells[2].
Erucin (0-50 μM, 24 h) induces the cleavage of PARP-1 at 50 µM, and increases p53 and p21 protein expression in A549 cells[2].
Erucin decreases LPS-induced production of NO, prostaglandin E2 (PGE2), TNF-α, IL-6 and IL-1β in RAW 264.7 cells[3].
Erucin decreases LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in RAW 264.7 cells[3].
Erucin inhibits LPS-induced activation of NFκB Signaling in RAW 264.7 cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: AsPC‐1
Concentration: 10, 30, and 100 μM
Incubation Time: 72 h
Result: Showed a significant and concentration‐dependent reduction of cell viability.

Cell Cycle Analysis[1]

Cell Line: AsPC‐1
Concentration: 30 μM
Incubation Time: 72 h
Result: Showed a particular increase of cells number in the G2/M phase (36.6% ± 3.5 vs. vehicle‐treated cells in the G2/M phase: 24.0% ± 1.3) and in the S‐phase (18.1% ± 1.5 vs. vehicle‐treated cells in the S phase: 11.0% ± 0.7) and a consequent significant reduction of cells in the G0/G1 phase (35.1% ± 5.0 vs. vehicle‐treated cells in the G0/G1 phase: 59.5% ± 1.8.

Apoptosis Analysis[1]

Cell Line: AsPC‐1
Concentration: 30 μM
Incubation Time: 72 h
Result: Significantly increased the number of total apoptotic cells (apoptotic dead cells and apoptotic live cells; vehicle: 17.7% ± 2.5 vs. Erucin: 28.7% ± 4.2).

Cell Proliferation Assay[2]

Cell Line: A549
Concentration: 0-200 µM
Incubation Time: 24 h
Result: Showed antiproliferative effect with an IC50 of 97.7 µM.

Western Blot Analysis[2]

Cell Line: A549
Concentration: 0-50 µM
Incubation Time: 24 h
Result: Induced the cleavage of PARP-1 at 50 µM. Increased p53 and p21 protein expression.

Western Blot Analysis[3]

Cell Line: RAW 264.7
Concentration: 0, 2.5, and 5 µM
Incubation Time: 30 min
Result: Decreased the expression of iNOS and COX-2 induced by LPS. Suppressed the LPS-induced reduction in IκB-α. Suppressed NFκB DNA binding and transcriptional activity.

RT-PCR[3]

Cell Line: RAW 264.7
Concentration: 0, 2.5, and 5 µM
Incubation Time: 24 h
Result: Decreased LPS-induced TNF-α, IL-6 and IL-1β production.
In Vivo

Erucin (ERU) (0-300 nM) significantly inhibits TPA-induced edema formation[3].
Erucin (30 μmol/kg; i.p.; twice a week for 4 week) shows neuroprotective effects[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female ICR mice (4 weeks of age), TPA (12-O-tetradecanoylphorbol-13-acetate)-induced mouse ear edema model[3]
Dosage: 0, 100, and 300 nM
Administration: Topically applied to the mouse ear 30 min prior to the topical application of TPA
Result: Significantly inhibited TPA-induced edema formation.
Animal Model: Male C57Bl/6 mice (9 weeks old, 25–30 g body weight)[4]
Dosage: 30 μmol/kg
Administration: Intraperitoneal administration, twice a week, 4 weeks (Induce brain lesion by intrastriatal injection of 6-OHDA)
Result: Induced a partial recovery in the rotational behavior test. Upregulated the expression of TH. Counteract neuronal death and DNA fragmentation in 6-OHDA lesioned mice. increase total GSH and Nrf2 levels in 6-OHDA lesioned mice.
Molecular Weight

161.29

Formula

C6H11NS2
CAS No.
SMILES

CSCCCCN=C=S
Shipping

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Erucin4430-36-8ApoptosisIsothiocyanateAsPC‐1A549Parkinson’s DiseaseRAW 264.7Inhibitorinhibitorinhibit

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