1. MCE Kits
  2. RT Master Mix for qPCR

RT Master Mix for qPCR 

Cat. No.: HY-K0510
Manual SDS

MCE RT Master Mix for qPCR is a convenient, ready-to-use formulation for reverse transcription and the cDNA product can be directly applied as a template in a standard PCR and real time quantitative PCR (qPCR).

RT Master Mix for qPCR
Size Price Stock Quantity
1  mL (100 rxns) USD 210 In-stock
Estimated Time of Arrival: December 31
5  mL (100 rxns x5) USD 940 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE RT Master Mix for qPCR is a convenient, ready-to-use formulation for reverse transcription. The 2× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis. With RNA added, the optimized system will provide sensitive and reliable cDNA synthesis. Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommended for detection of the expression levels of interested genes.

 

Features:

•   Only one transfer step reduces handling errors significantly.

•   Optimized buffer enhances sensitive and reliable cDNA synthesis.

•   Super-efficient reaction even with low template amounts (total RNA: 5 ng-5 μg in a 20 μL reaction).

•   The kit provides both Oligo dT Primer and Random Primer.

Storage

Stored at -20°C, and is stable for up to 1 year.

Avoid repetitive freeze-thaw cycles.

Protocol

1   Thaw RNA templates and the 2× Super RT Mix on ice. Mix solutions gently but thoroughly.

2   Prepare the following reaction mixture in a PCR tube on ice.

Components Quantity
2× Super RT Mix 10 μL
Total RNA / mRNA 5 ng-5 μg / 5 ng-500 ng
RNase-Free H2O To 20 μL

3   Mix the components well and collect by brief centrifugation. Incubate the mixture in a PCR instrument or water bath as follows:

Temperature Time
25ºC 10 mins
42ºC 30-60 mins
85ºC 2 mins

Note:

a.  For GC rich or structurally complex RNA templates, increasing the RT incubation temperature up to 50ºC may improve the yields of cDNA.

b.  Stop the reaction by heating at 85ºC for 2 mins followed by chilling on ice.

4   The newly synthesized first-strand cDNA is ready for immediate downstream applications or for long-term storage at -20ºC.

Components
Components HY-K0510-100 rxns HY-K0510-500 rxns
2× Super RT Mix 1 mL 1 mL×5
RNase-Free H2O 1 mL×2 1 mL×10
Documentation

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RT Master Mix for qPCR
Cat. No.:
HY-K0510
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