1. Academic Validation
  2. Thrombostatin, a bradykinin metabolite, reduces platelet activation in a model of arterial wall injury

Thrombostatin, a bradykinin metabolite, reduces platelet activation in a model of arterial wall injury

  • Cardiovasc Res. 2002 Mar;53(4):984-92. doi: 10.1016/s0008-6363(01)00514-4.
Alejandro R Prieto 1 Hongbao Ma Ruiping Huang Gauhar Khan Kenneth A Schwartz Elie E Hage-Korban Alvin H Schmaier John M Davis Ahmed A K Hasan George S Abela
Affiliations

Affiliation

  • 1 Department of Internal Medicine, Division of Cardiology, Michigan State University, East Lansing, MI 48824, USA.
Abstract

Objective: Thrombin activates platelets and contributes to the occlusion of arteries following thrombolytic therapy or angioplasty. Thrombostatin (RPPGF), the angiotensin converting Enzyme degradation product of bradykinin, inhibits alpha-thrombin induced platelet activation. We hypothesized that thrombostatin prevents platelet aggregation and adhesion after balloon angioplasty (BA).

Methods: Platelet-rich plasma (PRP) was obtained from 22 Beagle dogs before sacrifice and 10% of the PRP was labeled with 111In. Carotid arteries were then removed from each dog and mounted in a dual perfusion chamber and intimal injury was performed with BA. 111In-PRP with or without thrombostatin or aspirin alone was perfused through the arteries for 60 min. During perfusion, platelet volume was measured using a Coulter counter and a laser-light scattering technique. Platelet adhesion to arteries was measured by radioactivity count.

Results: Arterial injury alone compared to non-injury increased platelet volume in the circuit by 1.4 times (x) (P<0.05) using a Coulter counter or 1.8x (P<0.05) using laser-light scattering and increased platelet adhesion by 2.3x (P<0.01). When compared to BA injury alone, the addition of thrombostatin reduced platelet volume by 1.8x (P<0.03) as measured by Coulter counter or 1.9x (P<0.01) by laser-light scattering and platelet adhesion by 4.2x (P<0.05). Compared to BA injury alone, aspirin reduced platelet volume by 1.2x (P<0.01) as assessed by Coulter counter or 1.5x (P<0.03) using laser-light scattering and platelet adhesion by 1.8x (P<0.02).

Conclusion: Thrombostatin or aspirin independently decreases evidence of platelet activation in the canine carotid artery model of BA injury.

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