[Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism]

Aim: To study the effect of uvarigrin on mitochondrial dependent pathway during the Apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.

Methods: MTT assay was used to detect the cytotoxic effect of uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified uvarigrin-induced Apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of Reactive Oxygen Species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation.

Results: Uvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the uvarigrin-induced cells death was found to be due to Apoptosis, as determined by Annexin V FITC staining. During the Apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.

Conclusion: Uvarigrin induced Apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.