2. Academic Validation
  3. Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-α-L-amino-LNA FRET pairs

Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-α-L-amino-LNA FRET pairs

  • Chem Commun (Camb). 2013 Jan 18;49(5):511-3. doi: 10.1039/c2cc37547h.
I Kira Astakhova 1,  T Santhosh Kumar,  Meghan A Campbell,  Alexey V Ustinov,  Vladimir A Korshun,  Jesper Wengel
Affiliations

Affiliation

  • 1 Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, DK-5230 Odense M, Denmark. [email protected]
PMID: 23201901 DOI: 10.1039/c2cc37547h
Abstract

Novel pyrene-perylene α-L-LNA FRET pairs described herein effectively detect assembly of 2- and 3-way branched DNA nanostructures prepared by postsynthetic microwave-assisted CuAAC Click Chemistry. The fluorescent signalling of assembly by internally positioned FRET pairs is achieved with low to no fluorescence background signal, remarkably low limit of target detection values and stabilization of the resulting nanostructures.

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