2. Academic Validation
  3. DHTP is an allosteric inhibitor of the kinesin-13 family of microtubule depolymerases

DHTP is an allosteric inhibitor of the kinesin-13 family of microtubule depolymerases

  • FEBS Lett. 2014 Jun 27;588(14):2315-20. doi: 10.1016/j.febslet.2014.05.024.
Lama Talje 1,  Khaled Ben El Kadhi 2,  Kaleem Atchia 1,  Thierry Tremblay-Boudreault 1,  Sébastien Carreno 3,  Benjamin H Kwok 4
Affiliations

Affiliations

  • 1 Chemical Biology of Cell Division Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada.
  • 2 Cellular Mechanisms of Morphogenesis during Mitosis and Cell Motility Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada.
  • 3 Cellular Mechanisms of Morphogenesis during Mitosis and Cell Motility Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada; Département de Pathologie et de Biologie Cellulaire, Université de Montréal, Montréal, Québec H3C 3J7, Canada.
  • 4 Chemical Biology of Cell Division Laboratory, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal, Québec H3C 3J7, Canada; Département de médecine, Université de Montréal, Montréal, Québec H3C 3J7, Canada. Electronic address: [email protected].
PMID: 24859087 DOI: 10.1016/j.febslet.2014.05.024
Abstract

The kinesin-13 family of microtubule depolymerases is a major regulator of microtubule dynamics. RNA interference-induced knockdown studies have highlighted their importance in many cell division processes including spindle assembly and chromosome segregation. Since microtubule turnovers and most mitotic events are relatively rapid (in minutes or seconds), developing tools that offer faster control over protein functions is therefore essential to more effectively interrogate kinesin-13 activities in living cells. Here, we report the identification and characterization of a selective allosteric kinesin-13 inhibitor, DHTP. Using high resolution microscopy, we show that DHTP is cell permeable and can modulate microtubule dynamics in cells.

Keywords

Cell division; Chemical inhibitor; Enzyme; Kinesin; Microtubule.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-177898
    Kinesin-13 Inhibitor