2. Academic Validation
  3. Triterpenoid saponins from the roots of Clematis argentilucida

Triterpenoid saponins from the roots of Clematis argentilucida

  • Fitoterapia. 2014 Sep;97:234-40. doi: 10.1016/j.fitote.2014.06.015.
Mei Zhao 1,  Ning Ma 2,  Feng Qiu 3,  Xiangrong Tian 4,  Yan Zhang 5,  Haifeng Tang 6,  Xinyou Liu 7
Affiliations

Affiliations

  • 1 Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China; Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China.
  • 2 Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
  • 3 School of Traditional Chinese Medicines, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
  • 4 Research & Development Center of Biorational Pesticide, College of Plant Protection, Northwest A&F University, Yangling 712100, PR China.
  • 5 Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China.
  • 6 Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, PR China; Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China. Electronic address: [email protected].
  • 7 Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China. Electronic address: [email protected].
PMID: 24979221 DOI: 10.1016/j.fitote.2014.06.015
Abstract

Reinvestigation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of a new ursane-type triterpenoid saponin 1 and a new taraxerane-type saponin 2, four known saponins 3-6 first isolated from the species, together with seven saponins 7-13 reported in the previous papers. The structures of saponins 1-6 were elucidated by extensive spectroscopic analysis and chemical evidences. The ursane-type and taraxerane-type triterpenoid saponins were obtained from genus Clematis for the first time, and the aglycone of saponin 1, 3β,28-dihydroxy-18αH-ursan-20-en was first encountered. The cytotoxicity of all the saponins was evaluated against human glioblastoma U251MG cell lines. The monodesmosidic saponins 1, 2 and 4-8 exhibited cytotoxic activity against the cells with IC50 values ranging from 6.95 to 38.51 μM.

Keywords

Clematis argentilucida; Cytotoxicity; Ranunculaceae; Taraxerane-type; Triterpenoid saponin; Ursane-type.

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