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  3. Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

  • Cytotechnology. 2015 Dec;67(6):1059-66. doi: 10.1007/s10616-014-9746-8.
Dilek Akyıl 1,  Arzu Özkara 2,  S Feyza Erdoğmuş 3,  Yasin Eren 4,  Muhsin Konuk 5,  Esra Sağlam 6
Affiliations

Affiliations

  • 1 Department of Biology, Faculty of Arts and Sciences, Afyon Kocatepe University, Afyonkarahisar, Turkey. [email protected].
  • 2 Department of Biology, Faculty of Arts and Sciences, Afyon Kocatepe University, Afyonkarahisar, Turkey.
  • 3 Department of Veterinary Sciences, Bayat Vocational High School, Afyon Kocatepe University, Afyonkarahisar, Turkey.
  • 4 Department of Science, Faculty of Education, Suleyman Demirel University, Isparta, Turkey.
  • 5 Department of Molecular Biology and Genetics (English), Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey.
  • 6 Department of Pharmacology and Clinical Pharmacology, Üsküdar University, Istanbul, Turkey.
PMID: 25017922 DOI: 10.1007/s10616-014-9746-8
Abstract

This study evaluates the cytotoxic and genotoxic potential of alloxydim sodium using micronucleus (MN) assay, in human peripheral lymphocytes. MN assay was used to investigate the genotoxic effects of alloxydim sodium in human peripheral lymphocytes treated with 250, 500, 750, 1,000 µg/ml concentrations of alloxydim sodium for 24 and 48 h. Solvent, negative and positive controls were also used in the experiments in parallel. The obtained results were evaluated in statistical analyses by using Dunnett-t test (two sided) and p < 0.05 was accepted as significant. Alloxydim sodium significantly increased the MN formation compared with the negative control, at both 750 and 1,000 µg/ml concentrations and treatment periods. We also evaluated the nuclear division index (NDI) for cytotoxicity of this pesticide in the experiment, and finally observed a significant decrease of the NDI values at all concentrations of alloxydim sodium and at both treatment periods.

Keywords

Alloxydim sodium; Genotoxicity; MN frequency; Micronucleus assay; Nuclear division index.

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