2. Academic Validation
  3. Neuropharmacological effects of carvacryl acetate on δ-aminolevulinic dehydratase, Na+, K+-ATPase activities and amino acids levels in mice hippocampus after seizures

Neuropharmacological effects of carvacryl acetate on δ-aminolevulinic dehydratase, Na+, K+-ATPase activities and amino acids levels in mice hippocampus after seizures

  • Chem Biol Interact. 2015 Jan 25:226:49-57. doi: 10.1016/j.cbi.2014.12.001.
Lúcio Fernandes Pires 1,  Luciana Muratori Costa 2,  Antonia Amanda Cardoso de Almeida 2,  Oskar Almeida Silva 2,  Gilberto Santos Cerqueira 3,  Damião Pergentino de Sousa 4,  Rosana Martins Carneiro Pires 5,  Prabodh Satyal 6,  Rivelilson Mendes de Freitas 7
Affiliations

Affiliations

  • 1 Postgraduate Program of Pharmacology, Federal University of Piauí, Teresina, PI, Brazil. Electronic address: [email protected].
  • 2 Department of Biochemistry and Pharmacology, Laboratory of Experimental Neurochemistry Research, Center of Pharmaceutical Technology, Federal University of Piauí, Teresina, PI, Brazil.
  • 3 Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
  • 4 Department of Pharmaceutical Science, Federal University of Paraíba, Campus I, João Pessoa, PB, Brazil.
  • 5 Postgraduate Program of Food and Nutrition, Federal University of Piauí, Teresina, PI, Brazil.
  • 6 Chemistry Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
  • 7 Postgraduate Program of Pharmacology, Federal University of Piauí, Teresina, PI, Brazil; Department of Biochemistry and Pharmacology, Laboratory of Experimental Neurochemistry Research, Center of Pharmaceutical Technology, Federal University of Piauí, Teresina, PI, Brazil. Electronic address: [email protected].
PMID: 25490531 DOI: 10.1016/j.cbi.2014.12.001
Abstract

Epileptic syndromes are highly prevalent neurological conditions and can often be disabling. In order to find an alternative for treatment, this study evaluated anticonvulsant effects of carvacryl acetate (CA), a derivative of monoterpene carvacrol, after seizures induced by pilocarpine (P400), picrotoxin (PIC) or pentylenetetrazol (PTZ). We also analyzed the CA effects on Na+, K+-ATPase and δ-aminolevulinic acid dehydratase (δ-ALA-D) activities in hippocampus mice after seizures induced by P400, PIC or PTZ. In addition, glutamate, δ-aminobutyric acid (GABA), glutamine and aspartate levels in mice hippocampus treated with CA after seizures induced by P400, PIC or PTZ were also measured. CA produced anticonvulsant effects against seizures induced by P400, PIC or PTZ, and its effects were reversed by flumazenil, suggesting that action mechanism can be mediated by GABAergic system. CA increased GABA levels, but did not alter glutamate and aspartate concentrations in mice hippocampus after seizures induced by P400, PIC or PTZ when compared with seizures induced by P400, PIC or PTZ (p<0.05), respectively, as well as decreased glutamine content in mice hippocampus after seizures induced by PIC when compared with seizures induced by PIC (p<0.05). In addition, CA also increased Na+, K+-ATPase and δ-aminolevulinic acid dehydratase activities after seizures induced by P400, PIC or PTZ when compared with seizures induced by P400, PIC or PTZ (p<0.05), respectively. This study demonstrated that CA could be a future therapeutic option for treatment of epilepsy, with a multifactorial brain action mechanism.

Keywords

Acetate; Anticonvulsant; Carvacryl; Epilepsy; Mouse.

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