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  3. Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders

Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders

  • Methods Mol Biol. 2017;1517:223-237. doi: 10.1007/978-1-4939-6563-2_16.
Elena Poser 1,  Ilaria Genovese 1,  Silvia Masciarelli 2,  Teresa Bellissimo 2,  Francesco Fazi 3,  Gianni Colotti 4
Affiliations

Affiliations

  • 1 CNR-National Research Council of Italy, Institute of Molecular Biology and Pathology, Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy.
  • 2 Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology & Medical Embryology, Sapienza University of Rome, Via A. Scarpa 14-16, 00161, Rome, Italy.
  • 3 Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Section of Histology & Medical Embryology, Sapienza University of Rome, Via A. Scarpa 14-16, 00161, Rome, Italy. [email protected].
  • 4 IBPM - CNR Institute of Molecular Biology and Pathology, Italian National Research Council, Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, 00185, Rome, Italy. [email protected].
PMID: 27924486 DOI: 10.1007/978-1-4939-6563-2_16
Abstract

Surface plasmon resonance (SPR) is one of the most important techniques for the detection and the characterization of molecular interactions. SPR technology is a label-free approach for monitoring biomolecular interactions in real time. The binding of analytes to molecules immobilized on a thin metal film (ligand) determines a change in the refractive index and, therefore in the angle of extinction of LIGHT, is reflected when polarized LIGHT hits the film, monitored in real time as a change in the position of the dip in reflected intensity. Since SPR detects mass, the technique is label-free.Here, we describe the use of SPR techniques to study the interaction between Argonaute 2 and small molecular compounds selected by means of high-throughput docking screening.

Keywords

Analyte; Ligand; Protein-inhibitor interaction; Small molecules; Surface plasmon resonance.

Figures
Products
  • Cat. No.
    Product Name
    Description
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  • HY-119024
    ≥99.0%, AGO2 Inhibitor