2. Academic Validation
  3. Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine

Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine

  • Sci Rep. 2017 Mar 8;7:43796. doi: 10.1038/srep43796.
Adila Aipire 1,  Jinyu Li 1,  Pengfei Yuan 1,  Jiang He 2,  Yelang Hu 1,  Lu Liu 3,  Xiaoli Feng 3,  Yijie Li 1,  Fuchun Zhang 1,  Jianhua Yang 1 4,  Jinyao Li 1
Affiliations

Affiliations

  • 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
  • 2 Key laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, 140 Xinhua South Road, Urumqi 830004, China.
  • 3 XinJiang DingJu Biotech CO., LTD, 181 Xicai Road, Urumqi 830000, China.
  • 4 Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, TX 77030, USA.
PMID: 28272545 DOI: 10.1038/srep43796
Abstract

Licorice has been used as herbal medicine and natural sweetener. Here, we prepared Glycyrrhiza uralensis water extract (GUWE) and investigated the effect of GUWE on the maturation and function of dendritic cells (DCs) and its adjuvant effect on DC-based vaccine. We observed that GUWE dose-dependently promoted DC maturation and cytokine secretion through TLR4 signaling pathway. The capacity of DC to stimulate allogenic splenocyte proliferation was also enhanced by GUWE treatment. Compared with control group, GUWE treated DCs pulsed with human papillomavirus (HPV)-16 E6/E7 Peptides significantly inhibited the tumor growth in both early and late therapeutic groups. In early therapeutic group, the frequencies of induced regulatory T cells (iTregs: CD4+CD25-Fopx3+) and CD4+ and CD8+ T cells were significantly decreased and increased, respectively. HPV-16-specific CD8+ T cell responses were significantly induced and negatively correlated with iTreg frequencies and tumor weight. These results indicated the immunoregulatory activities of licorice.

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