2. Academic Validation
  3. Acute and subchronic toxicity of pyraclostrobin in zebrafish (Danio rerio)

Acute and subchronic toxicity of pyraclostrobin in zebrafish (Danio rerio)

  • Chemosphere. 2017 Dec:188:510-516. doi: 10.1016/j.chemosphere.2017.09.025.
Cheng Zhang 1,  Jun Wang 2,  Shuai Zhang 3,  Lusheng Zhu 4,  Zhongkun Du 5,  Jinhua Wang 6
Affiliations

Affiliations

  • 1 College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian, 271018, PR China. Electronic address: [email protected].
  • 2 College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian, 271018, PR China. Electronic address: [email protected].
  • 3 College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian, 271018, PR China. Electronic address: [email protected].
  • 4 College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian, 271018, PR China. Electronic address: [email protected].
  • 5 College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian, 271018, PR China. Electronic address: [email protected].
  • 6 College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian, 271018, PR China. Electronic address: [email protected].
PMID: 28910725 DOI: 10.1016/j.chemosphere.2017.09.025
Abstract

The aim of the present study was to assess the toxic effects of pyraclostrobin on DNA damage and antioxidant enzymatic activities in the zebrafish (Danio rerio) liver. Based on the 96-h median lethal concentration (96 h LC50, 0.056 mg/L) of this chemical, fish were exposed to three doses (0.001, 0.01, and 0.02 mg/L) and sampled on days 7, 14, 21 and 28 after the initiation of a subchronic toxicity test. The levels of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), glutathione S-transferase (GST), Reactive Oxygen Species (ROS) and DNA damage were determined. The amount of pyraclostrobin residue in the water was also measured. The concentrations in the three treatment groups varied no more than 5% during the exposure periods, indicating that pyraclostrobin is relatively stable during this time in an aquatic environment. ROS and MDA levels significantly changed in a dose dependent manner during the experiment. Enzymatic activities were inhibited to a certain extent. DNA damage was significantly enhanced. These results collectively indicate that pyraclostrobin induces oxidative stress and DNA damage in zebrafish.

Keywords

96 h LC(50); Antioxidant enzyme; DNA damage; Genetic toxicity; Reactive oxygen species; Residue analysis.

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